BINDING OF BACTERIA FROM THE GENUS BRUCELLA TO HUMAN LYMPHOCYTES-B
BINDING OF BACTERIA FROM THE GENUS BRUCELLA TO HUMAN LYMPHOCYTES-B
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DOI：
10.1128/iai.31.2.816-821.1981
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发表时间：
1981-01-01
影响因子：
3.1
通讯作者：
TEODORESCU, M
中科院分区：
文献类型：
--
作者：
BRATESCU, A;MAYER, EP;TEODORESCU, M
不同的淋巴细胞亚群结合不同属和种的不同菌株;在这些菌株中，有一种菌株为B。与所有人B淋巴细胞结合的羊种血吸虫。以确定是否与B结合。melitensis对人B淋巴细胞是一种株、种或属的特性，结合B。melitensis，B. abortus，牛种B. ovis，B. suis，B.犬和B.对人正常和白血病B淋巴细胞进行了试验。不同种布鲁氏菌的结合。通过单标记和双标记实验测定对B淋巴细胞的特异性，其中用抗L链抗体包被的大肠杆菌菌株用作B细胞的标记。B。melitensis和抗体包被E.与相同的细胞结合。所有其他测试的细菌都与正常或白血病的B淋巴细胞结合。B。ovis和B.非人类病原体的新瘤比人类病原体B结合更少的B淋巴细胞。abortus，牛种B. melitensis，B. suis和B.犬。非致病菌与淋巴细胞形成的淋巴细胞的性质，即，每淋巴细胞的细菌数低于致病性布鲁氏菌。因此，所有的布鲁氏菌属物种（Brucella spp.）测试的抗体具有结合人B淋巴细胞的能力，但只有那些是人病原体的抗体才能牢固地结合所有的B淋巴细胞，并可用作这些细胞的可靠标记。布鲁氏菌属的结合。对B淋巴细胞的影响可能与人类布鲁氏菌病的发病机制有关。
Various lymphocyte subpopulations bind different strains of bacteria of different genera and species; among these, there is a strain of B. melitensis which binds to all human B lymphocytes. To determine whether the binding of B. melitensis to human B lymphocytes is a strain, species or genus characteristic, the binding of B. melitensis, B. abortus, B. ovis, B. suis, B. canis and B. neotomae to human normal and leukemic B lymphocytes was tested. The binding of different Brucella spp. to B lymphocytes was determined by single- and double-labeling experiments in which a strain of Escherichia coli, coated with anti-L chain antibodies, was used as a marker for B cells. B. melitensis and antibody-coated E. coli bound to the same cells. All the other species of bacteria tested bound to the B lymphocytes, normal or leukemic. B. ovis and B. neotomae, which are not human pathogens, bound to fewer B lymphocytes than did the human pathogens B. abortus, B. melitensis, B. suis and B. canis. The quality of rosettes formed by the nonpathogenic bacteria with the lymphocytes, i.e., the number of bacteria per lymphocytes, was lower than that of pathogenic Brucella spp. Thus, all of the Brucella spp. tested have the ability to bind to human B lymphocytes, but only those which are human pathogens bind firmly to all B lymphocytes and may be used as reliable markers for these cells. The binding of Brucella spp. to B lymphocytes may have some bearing on the pathogenesis of brucellosis in humans.