Crystallization and preliminary X-ray diffraction analysis of the cytosolic domain of the Mg2+ transporter MgtE.
Crystallization and preliminary X-ray diffraction analysis of the cytosolic domain of the Mg2+ transporter MgtE.
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DOI：
10.1107/s1744309107032344
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发表时间：
2007-08
期刊：
影响因子：
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通讯作者：
Yoshiki Tanaka;M. Hattori;S. Fukai;Ryuichiro Ishitania;O. Nureki
中科院分区：
文献类型：
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作者：
Yoshiki Tanaka;M. Hattori;S. Fukai;Ryuichiro Ishitania;O. Nureki
Mg（2+）转运蛋白的MgtE家族在生命的所有领域中普遍保守。MgtE Mg（2+）转运蛋白的胞质结构域包括已知在转运蛋白中发挥调节功能的胱硫醚-β-合酶（CBS）结构域。在Mg（2+）存在和不存在的情况下，嗜热栖热菌MgtE的胞质结构域被过表达、纯化和结晶。在Mg（2+）存在下形成的晶体使用同步辐射衍射X射线至2.3 A分辨率，属于空间群P6（5）22，晶胞参数a = B = 57.7，c = 317.6 A，并且预期在不对称单元中包含一个分子。在不存在Mg（2+）的情况下形成的晶体使用同步辐射衍射X射线至3.5 A分辨率，属于空间群P2（1）2（1）2（1），晶胞参数a = 77.0，B = 100.3，c = 100.3 A，并且预期在不对称单元中包含两个分子。
The MgtE family of Mg(2+) transporters is ubiquitously conserved in all domains of life. The cytosolic domains of the MgtE Mg(2+) transporters include a cystathionine-beta-synthase (CBS) domain which is known to play a regulatory function in transporter proteins. The cytosolic domain of MgtE from Thermus thermophilus was overexpressed, purified and crystallized in the presence and absence of Mg(2+). The crystals formed in the presence of Mg(2+) diffracted X-rays to 2.3 A resolution using synchrotron radiation, belong to space group P6(5)22 with unit-cell parameters a = b = 57.7, c = 317.6 A and are expected to contain one molecule in the asymmetric unit. The crystals formed in the absence of Mg(2+) diffracted X-rays to 3.5 A resolution using synchrotron radiation, belong to space group P2(1)2(1)2(1) with unit-cell parameters a = 77.0, b = 100.3, c = 100.3 A and are expected to contain two molecules in the asymmetric unit.