A forward reconstruction, holographic method to overcome the lens effect during 3D detection of semi-transparent, non-spherical particles
A forward reconstruction, holographic method to overcome the lens effect during 3D detection of semi-transparent, non-spherical particles
复制标题
一种前向重建全息方法，用于克服半透明非球形颗粒 3D 检测过程中的透镜效应
DOI：
10.1039/d2sm00738j
复制
发表时间：
2022
期刊：
影响因子：
3.4
通讯作者：
Narsimhan, Vivek
中科院分区：
文献类型：
--
作者：
Tai, Cheng-Wei;Ahmadzadegan, Adib;Ardekani, Arezoo;Narsimhan, Vivek
半透明颗粒如聚苯乙烯微粒的悬浮液通常用作微流变学、生物学和微流体学研究中的模型系统。全息术是一种有价值的工具，它允许人们获得颗粒位置和取向的3-D信息，但是前向重建技术通常难以准确地推断具有O（1）纵横比的半透明球体的该信息，因为来自颗粒的透镜效应引入了复杂的图案。我们提出了一种重建方法，利用图像矩信息产生一个掩模的清晰图案的透镜效应，并给出合理的估计的3-D的位置和方向的颗粒。在这项工作中提出的方法使用的平均颗粒的几何信息，以确定工艺参数，并确定适当的检测区域。zc的平均检测误差小于平均颗粒厚度的25%，面内和面外取向的平均误差分别为2°和4°。我们的方法在检测粒子质心（xc，yc，zc）和面内取向方面提供了与Byeon等人（H. Byeon，T.去和S。J. Lee，应用选择，2016，54，2106-2112; H. Byeon，T.去和S。J. Lee，Opt. Express，2016，24，598-610）。该方法为确定粒子的面外倾角θ提供了一个明确定义的框架。最后，我们通过分析E.大肠杆菌细胞的全息影像。
Suspensions of semi-transparent particles such as polystyrene microparticles are commonly used as model systems in the study of micro-rheology, biology, and microfluidics. Holography is a valuable tool that allows one to obtain 3-D information for particle position and orientation, but forward reconstruction techniques often struggle to infer this information accurately for semi-transparent spheroids with an O(1) aspect ratio, since the lens effect from the particle introduces complex patterns. We propose a reconstruction method that uses image moment information to generate a mask over the sharp patterns from the lens effect and gives reasonable estimation of the 3-D position and orientation of the particle. The method proposed in this work uses the average particle geometry information to determine the process parameters and identify the appropriate detection zone. The average detection error for zc is less than 25% of the average particle thickness, and the average errors in the in-plane and out-of-plane orientations ϕ and θ are 2° and 4°, respectively. Our method provides comparable accuracy in the detection of the particle center of mass (xc, yc, zc) and in-plane orientation ϕ as a recent forward reconstruction method for semi-transparent particles proposed by Byeon et al. (H. Byeon, T. Go and S. J. Lee, Appl. Opt., 2016, 54, 2106–2112; H. Byeon, T. Go and S. J. Lee, Opt. Express, 2016, 24, 598–610). This method provides a clearly defined framework for identifying the particle's out-of-plane tilt angle θ. We finally demonstrate the applicability of the method to opaque, slender (aspect ratio AR ≫ 1) particles by analyzing the 3-D motion of E. coli cells from holographic video footage.