Clinicopathological implications of NQO1 overexpression in the prognosis of pancreatic adenocarcinoma.
Clinicopathological implications of NQO1 overexpression in the prognosis of pancreatic adenocarcinoma.
复制标题
NQO1过表达对胰腺癌预后的临床病理学意义
DOI：
10.3892/ol.2017.5821
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发表时间：
2017-05
期刊：
影响因子：
2.9
通讯作者：
Lin Z
中科院分区：
文献类型：
--
作者：
Ji M;Jin A;Sun J;Cui X;Yang Y;Chen L;Lin Z
烟酰胺腺嘌呤二核苷酸磷酸：醌氧化还原酶1(NQO1)保护细胞免受氧化损伤。据报道，NQO1在许多实体肿瘤中表达上调，提示NQO1在肿瘤的发生和发展中发挥作用。本研究旨在探讨NQO1在胰腺导管腺癌(PDAC)中过度表达的临床预后意义。应用免疫组织化学方法检测181例胰腺癌组织中NQO1蛋白的表达，其中PDAC 126例，正常胰腺55例。免疫荧光染色鉴定NQO1蛋白在胰腺癌BxPC-3细胞中的亚细胞定位。用χ-2和Fisher‘s Exact检验评价NQO_1过表达与临床特征的关系。采用Kaplan-Meier分析计算PDAC患者的总存活率。采用COX比例风险回归模型进行单因素和多因素分析。NQO1蛋白主要定位于BxPC-3细胞的胞浆和胞核。胰腺癌组织中NQO1强阳性表达(65.9%，83/126)明显高于正常胰腺组织(10.9%，6/55)。NQO1蛋白阳性率与肿瘤分级、淋巴结分期、肿瘤转移(TNM)分期有关。多因素分析显示，NQO1是影响PDAC预后的独立因素，与TNM分期有关。总之，NQO1的高表达似乎与PDAC的进展有关，并且可能是PDAC的一个独立的预后生物标志物。
Nicotinamide adenine dinucleotide phosphate: quinone oxidoreductase 1 (NQO1) protects cells from oxidative damage. NQO1 has been reported to be upregulated in numerous solid tumors, suggesting a role in carcinogenesis and tumor progression. The present study attempted to investigate the clinical prognostic significance of NQO1 overexpression in pancreatic ductal adenocarcinoma (PDAC). A total of 181 tissue specimens were studied, including 126 PDAC and 55 normal pancreas specimens, which were selected for immunohistochemical staining of NQO1 protein. Immunofluorescence staining was additionally performed to identify the subcellular localization of NQO1 protein in pancreatic cancer BxPC-3 cells. The association between NQO1 overexpression and the clinical features of PDAC were evaluated by χ2 and Fisher's exact test. Overall survival of PDAC patients was calculated using Kaplan-Meier analysis. Univariate and multivariate analyses were performed using the Cox proportional hazards regression model. The NQO1 protein was mainly located in the cytoplasm and nucleus of BxPC-3 cells. The strongly positive rate of NQO1 expression in PDAC (65.9%, 83/126) was increased compared with that in normal pancreatic tissues (10.9%, 6/55). The positive rate of NQO1 protein was associated with grading, lymph node stage and tumor-node-metastasis (TNM) stage. Additionally, multivariate analysis suggested that NQO1 was a significant independent prognostic factor along with TNM stage in PDAC. In conclusion, high expression of NQO1 appears to be associated with PDAC progression, and may be an independent prognostic biomarker in PDAC.