Expression of aquaporin1, a water channel protein, in cytoplasm is negatively correlated with prognosis of breast cancer patients.
Expression of aquaporin1, a water channel protein, in cytoplasm is negatively correlated with prognosis of breast cancer patients.
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细胞质中水通道蛋白aquaporin1的表达与乳腺癌患者的预后呈负相关。
DOI：
10.18632/oncotarget.6994
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发表时间：
2016-02-16
期刊：
影响因子：
--
通讯作者：
Ma Y
中科院分区：
文献类型：
--
作者：
Qin F;Zhang H;Shao Y;Liu X;Yang L;Huang Y;Fu L;Gu F;Ma Y
水通道蛋白1（Aquaporin1，AQP1）是一个高度保守的水通道蛋白家族，其功能是促进水分子通过细胞膜。虽然越来越多的证据表明AQP1的胞浆定位是重要的，但AQP1的胞浆分布所对应的功能迄今为止还很少被探索。在我们的临床研究中，我们首次报道了AQP1主要定位于浸润性乳腺癌患者癌细胞的胞浆中，胞浆中的AQP1是一个独立的预后因素。AQP1高表达者生存期短，尤以管腔型为甚。此外，与我们在临床上的发现一致，在原代培养的乳腺癌细胞和AQP1过表达细胞系中进一步验证了AQP1的胞质表达，其中胞质AQP1的功能重要性在体外得到证实。总之，我们的研究提供了第一个证据表明，AQP1的细胞质表达促进乳腺癌的进展，它可能是一个潜在的预后生物标志物乳腺癌。
Aquaporin1 (AQP1) belongs to a highly conserved family of aquaporin proteins which facilitate water flux across cell membranes. Although emerging evidences indicated the cytoplasm was important for AQP1 localization, the function of AQP1 corresponding to its cytoplasmic distribution has rarely been explored until present. In our clinical study, we reported for the first time that AQP1 was localized dominantly in the cytoplasm of cancer cells of invasive breast cancer patients and cytoplasmic AQP1 was an independent prognostic factor. High expression of AQP1 indicated a shorter survival, especially in luminal subtype. Moreover, in line with our findings in clinic, cytoplasmic expression of AQP1 was further validated in both primary cultured breast cancer cells and AQP1 over-expressing cell lines, in which the functional importance of cytoplasmic AQP1 was confirmed in vitro. In conclusion, our study provided the first evidence that cytoplasmic expression of AQP1 promoted breast cancer progression and it could be a potential prognostic biomarker for breast cancer.