Influence of cetyltrimethyl ammonium bromide on nutrient uptake and cell responses of Chlorella vulgaris.
Influence of cetyltrimethyl ammonium bromide on nutrient uptake and cell responses of Chlorella vulgaris.
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DOI：
10.1016/j.aquatox.2013.04.010
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发表时间：
2013-08
影响因子：
4.5
通讯作者：
Z. Liang;Fei Ge;Hui Zeng;Yin Xu;Fang Peng;Minghung Wong
中科院分区：
文献类型：
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作者：
Z. Liang;Fei Ge;Hui Zeng;Yin Xu;Fang Peng;Minghung Wong
藻类对营养物质的去除被认为是废水处理中的重要环节，但在这个生物过程中，一些有毒化学物质会抑制藻细胞的活性。研究了季铵盐类化合物(QAC)的代表十六烷基三甲基溴化铵(CTAB)对小球藻(Chlorella Vallgaris)对氨氮(NH4+)和总磷(TP)的吸收及藻细胞对CTAB胁迫的响应。当十六烷基三甲基溴化铵浓度从0增加到0.6 mg/L时，对NH4+和TP的吸收效率分别从88%和96%下降到18%和15%。藻细胞的反应表现出光合作用活性的下降，这表明从2.9a.u开始，叶绿素的自发荧光增加。细胞存活率从88%下降到51%；二醋酸酯荧光素染色细胞的荧光强度从71.5a.u下降，表明酯酶活性下降。到4.7a.u。此外，CTAB刺激的藻细胞大分子组成中PO峰和酰胺II峰的增强也证实了转录和翻译反应。结果表明，废水中的QAC可能通过降低藻细胞活性而显著抑制藻类对营养物质的吸收。
The removal of nutrients by algae is regarded as a vital process in wastewater treatment, however algal cell activity can be inhibited by some toxic chemicals during the biological process. This study investigated the uptake of ammonia nitrogen (NH4+) and total phosphorus (TP) by a green alga (Chlorella vulgaris) and algal cell responses under the stress of cetyltrimethyl ammonium bromide (CTAB), a representative for quaternary ammonium compounds (QACs, cationic surfactants). When the concentration of CTAB increased from 0 to 0.6mg/L, the uptake efficiencies of NH4+and TP decreased from 88% to 18% and from 96% to 15%, respectively. Algal cell responses showed a decline in photosynthesis activity as indicated by the increase of chlorophyll autofluorescence from 2.9a.u. to 25.3a.u.; and a decrease of cell viability from 88% to 51%; and also a drop in esterase activity as indicated by the decrease in fluorescence of fluorescein diacetate stained cells from 71.5a.u. to 4.7a.u. Additionally, a transcription and translation response was confirmed by an enhancement of PO peak and amide II peak in algal cellular macromolecular composition stimulated by CTAB. The results suggest that QACs in wastewater may inhibit nutrient uptake by algae significantly through declining algal cell activities.