Impact of Antifouling PEG Layer on the Performance of Functional Peptides in Regulating Cell Behaviors
Impact of Antifouling PEG Layer on the Performance of Functional Peptides in Regulating Cell Behaviors
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防污 PEG 层对功能肽调节细胞行为性能的影响
DOI：
10.1021/jacs.9b07105
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发表时间：
2019-10-23
影响因子：
15
通讯作者：
Liu, Runhui
中科院分区：
文献类型：
--
作者：
Chen, Qj;Yu, Shan;Liu, Runhui
细胞粘附肽和其他功能肽（如RGD、KRSR、YIGSR、VAPG和BMP-2肽）被广泛研究并用于组织工程支架和生物医学装置以调节细胞功能。虽然PEG经常被用作包被层，但它如何影响功能肽的性能尚不清楚。通过分析短链（OEG 4），中链（OEG 8）和长链（PEG 2K）PEG的影响，我们发现PEG链长是关键的，中等长度的PEG使功能肽能够在细胞粘附，迁移和分化中显示其最佳和真正的功能，通过提供优异的粘附性来最大限度地减少不需要的细胞粘附的背景噪声和足够高的功能肽表面密度。我们的研究结果为最大限度地发挥肽的真正功能提供了新的途径。本研究还提供了一种解决方案，以防止异质性，甚至分歧的结果所造成的不适当的选择，并提供了一个一般性的指导，在确定新的功能肽。
Cell adhesive and other functional peptides (such as RGD, KRSR, YIGSR, VAPG, and BMP-2 peptides) are extensively studied and utilized in tissue engineering scaffolds and biomedical devices to modulate cell functions. Though PEG is frequently used as the antifouling layer, it is unclear how it affects the performance of functional peptides. By analyzing the impact of PEG at short (OEG4), medium (OEG8), and long chain length (PEG2K), we reveal that PEG chain length is critical and a medium-length PEG enables functional peptides to display their optimal and genuine functions in cell adhesion, migration, and differentiation by providing excellent antifouling to minimize background noise of unwanted cell adhesion and high enough surface density of functional peptides. Our result provides new avenues for maximizing the genuine functions of peptides. This study also provides a solution to prevent the heterogeneous and even divergent results caused by inappropriate choice of antifouling PEG and provides a general guidance in identifying new functional peptides.