Long-term restoration of immunity against Epstein-Barr virus infection by adoptive transfer of gene-modified virus-specific T lymphocytes
Long-term restoration of immunity against Epstein-Barr virus infection by adoptive transfer of gene-modified virus-specific T lymphocytes
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DOI：
10.1038/nm0596-551
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发表时间：
1996-05-01
期刊：
影响因子：
82.9
通讯作者：
Rooney, CM
中科院分区：
文献类型：
--
作者：
Heslop, HE;Ng, CYC;Rooney, CM
抗原特异性细胞毒性T淋巴细胞（CTL）的连续转移为某些病毒感染提供了安全有效的治疗，并可证明在根除肿瘤细胞方面是有用的。输注的T细胞是否持续较长时间，保持其响应抗原刺激而扩增的能力尚不清楚。我们现在报告在有发展为EBV淋巴增生性疾病风险的免疫功能低下患者中长期检测基因标记的EB病毒（EBV）特异性CTL。输注CTL不仅恢复了针对EB病毒的细胞免疫反应，而且还建立了可以对病毒体内或离体挑战做出长达18个月反应的CTL前体群体。我们的研究结果支持抗原特异性CTL在过继免疫治疗中的更广泛使用。
Adoptive transfer of antigen-specific cytotoxic T lymphocytes (CTLs) offers safe and effective therapy for certain viral infections and could prove useful in the eradication of tumor cells. Whether or not the infused T cells persist for extended periods, retaining their ability to expand in response to antigenic stimulation, is not known. We now report long-term detection of gene-marked Epstein-Barr virus (EBV)-specific CTLs in immunocompromised patients at risk for the development of EBV lymphoproliferative disease. Infusions of CTLs not only restored cellular immune responses against EBV, but also established populations of CTL precursors that could respond to in vivo or ex vivo challenge with the virus for as long as 18 months. Our findings support wider use of antigen-specific CTLs in adoptive immunotherapy.