Comparison between radiation-induced cell cycle delay in lymphocytes and radiotherapy response in head and neck cancer.
Comparison between radiation-induced cell cycle delay in lymphocytes and radiotherapy response in head and neck cancer.
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DOI：
10.1038/bjc.1998.103
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发表时间：
1998-02
影响因子：
8.8
通讯作者：
Lewensohn, R
中科院分区：
文献类型：
--
作者：
Tell, R;Heiden, T;Granath, F;Borg, A L;Skog, S;Lewensohn, R
进行了一项研究，评估使用辐射诱导的淋巴细胞细胞周期延迟来预测肿瘤对放射治疗的反应。从49例头颈癌患者放疗前的全血和25例健康献血员中分离外周血淋巴细胞。在治疗后0-2个月评估对放射治疗的临床反应。在有丝分裂原刺激淋巴细胞后，使用流式细胞术测量辐射诱导的细胞周期延迟的水平。对10名正常供体的分析显示，试验内和供体内样本之间的变异性无显著差异。然而，来自这些健康供体的淋巴细胞的细胞周期数据显示G2期蓄积存在显著的个体间差异。放疗无效患者的S期细胞水平高于部分缓解者（P < 0.001）和完全缓解者（P = 0.016）。当分析G2中的细胞分数时，发现了反比关系（分别为P = 0.009和0.034）。一般而言，健康供体与无应答者相比具有相似的细胞周期动力学。总之，该结果表明，放射诱导的淋巴细胞细胞周期延迟与头颈部癌症患者对放射治疗的肿瘤反应呈负相关。然而，由于试验变异性和组间重叠，本试验用于预测个体肿瘤缓解的价值有限。
A study was made evaluating the use of radiation-induced cell cycle delay in lymphocytes to predict tumour response to radiotherapy. Peripheral blood lymphocytes were isolated from whole blood from 49 patients with head and neck cancer before treatment with radiotherapy and from 25 healthy donors. The clinical response to radiotherapy was assessed at 0-2 months after treatment. The level of radiation-induced cell cycle delay was measured using flow cytometry after mitogen stimulation of lymphocytes. The analysis of ten normal donors gave no significant difference in variability between the intra-assay and the intra-donor samples. However, the cell cycle data for lymphocytes from these healthy donors showed significant inter-individual differences in G2 phase accumulation. Patients showing no response to radiotherapy had a high level of S-phase cells compared with partial (P < 0.001) and complete responders (P = 0.016). An inverse relationship was found when analysing the fraction of cells in G2 (P = 0.009 and 0.034 respectively). In general, healthy donors had similar cell cycle kinetics compared with the non-responders. In conclusion, the result indicates that radiation-induced cell cycle delay in lymphocytes is inversely correlated with tumour response to radiotherapy in head and neck cancer patients. However, the value of the present test for predicting individual tumour response is limited, because of assay variability and overlap between groups.