Adaptor functions of the Ca2+-binding protein ALG-2 in protein transport from the endoplasmic reticulum.
Adaptor functions of the Ca2+-binding protein ALG-2 in protein transport from the endoplasmic reticulum.
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Ca2+结合蛋白ALG-2在内质网蛋白转运中的衔接子功能。
DOI：
10.1080/09168451.2018.1525274
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Shibata H
中科院分区：
文献类型：
--
作者：
Zhang Wei;Muramatsu Ayaka;Matsuo Rina;Teranishi Naoki;Kahara Yui;Takahara Terunao;Shibata Hideki;Maki Masatoshi;Shibata H
凋亡相关基因2 （ALG-2）是一种Ca2+结合蛋白，具有5个重复的EF-hand基元，称为五EF-hand （PEF）结构域。它与多种靶蛋白相互作用，并在多种细胞活动中作为Ca2+依赖性适配器发挥作用。在细胞质中，通过与Sec31A的相互作用，ALG-2主要定位于内质网（ER）的一个特殊区域，称为内质网出口位点（ERES）。Sec31A是外壳蛋白复合物II （COPII）的外层外壳蛋白，从细胞质募集到ERES，形成COPII包裹的运输囊泡。我将概述目前关于ALG-2在调节ERES中Sec31A定位的生理意义的知识，以及ALG-2的以下接头功能，包括桥接Sec31A和膜联蛋白A11以稳定ERES中的Sec31A，聚合trk融合基因（TFG）产物，连接mapk1相互作用和纺锤体稳定（MISS）样（MISSL）和微管相关蛋白1B （MAP1B）以促进ER的顺行运输。
Apoptosis-linked gene 2 (ALG-2) is a Ca2+-binding protein with five repetitive EF-hand motifs, named penta-EF-hand (PEF) domain. It interacts with various target proteins and functions as a Ca2+-dependent adaptor in diverse cellular activities. In the cytoplasm, ALG-2 is predominantly localized to a specialized region of the endoplasmic reticulum (ER), called the ER exit site (ERES), through its interaction with Sec31A. Sec31A is an outer coat protein of coat protein complex II (COPII) and is recruited from the cytosol to the ERES to form COPII-coated transport vesicles. I will overview current knowledge of the physiological significance of ALG-2 in regulating ERES localization of Sec31A and the following adaptor functions of ALG-2, including bridging Sec31A and annexin A11 to stabilize Sec31A at the ERES, polymerizing the Trk-fused gene (TFG) product, and linking MAPK1-interacting and spindle stabilizing (MISS)-like (MISSL) and microtubule-associated protein 1B (MAP1B) to promote anterograde transport from the ER.