Induction of Gametogenesis in the Cnidarian Endosymbiosis Model Aiptasia sp.
Induction of Gametogenesis in the Cnidarian Endosymbiosis Model Aiptasia sp.
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DOI：
10.1038/srep15677
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发表时间：
2015-10-26
影响因子：
4.6
通讯作者：
Guse A
中科院分区：
文献类型：
--
作者：
Grawunder D;Hambleton EA;Bucher M;Wolfowicz I;Bechtoldt N;Guse A
内共生现象在刺胞动物中广泛存在，具有高度的生态学意义。热带海葵（Aiptasia sp.）是造礁珊瑚与共生藻属光合鞭毛藻内共生的实验室模型系统。在这里，我们确定了关键的环境线索，以诱导繁殖产卵在控制实验室条件下，在亚洲。我们发现，用蓝色波长的光模拟月球周期对于促进丰富的配子产生和同步释放是必要的。有性繁殖率是遗传决定的，在相似条件下，无性系之间存在差异。我们还发现无性繁殖率的差异是相反的。这项研究为进一步发展无肢动物模型系统提供了必要的基础，允许在实验室中分析基本的细胞和分子机制，以及研究与生态和进化相关的广泛问题。
Endosymbiosis is widespread among cnidarians and is of high ecological relevance. The tropical sea anemone Aiptasia sp. is a laboratory model system for endosymbiosis between reef-building corals and photosynthetic dinoflagellate algae of the genus Symbiodinium. Here we identify the key environmental cues to induce reproducible spawning in Aiptasia under controlled laboratory conditions. We find that simulating a lunar cycle with blue-wavelength light is necessary to promote abundant gamete production and synchronous release in well-fed animals. Sexual reproduction rates are genetically determined and differ among clonal lines under similar conditions. We also find the inverse difference in rates of asexual reproduction. This study provides the requisite basis for further development of the Aiptasia model system, allowing analysis of basic cellular and molecular mechanisms in the laboratory as well as investigations of broad questions of ecological and evolutionary relevance.