Expression of CRYM in different rat organs during development and its decreased expression in degenerating pyramidal tracts in amyotrophic lateral sclerosis
Expression of CRYM in different rat organs during development and its decreased expression in degenerating pyramidal tracts in amyotrophic lateral sclerosis
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CRYM在发育过程中大鼠不同器官中的表达及其在肌萎缩侧索硬化症退化锥体束中表达的减少
DOI：
10.1111/neup.12466
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发表时间：
2018
期刊：
影响因子：
2.3
通讯作者：
Iwaki Toru
中科院分区：
文献类型：
--
作者：
Hommyo Reiji;Suzuki Satoshi O.;Abolhassani Nona;Hamasaki Hideomi;Shijo Masahiro;Maeda Norihisa;Honda Hiroyuki;Nakabeppu Yusaku;Iwaki Toru
蛋白质μ晶体蛋白（μ M）是有袋动物透镜的一种新组分，具有两种功能：它是甲状腺激素转运的关键调节剂和含硫环酮亚胺的还原酶。在这项研究中，我们研究的变化，在不同的大鼠器官发育过程中，免疫组化和免疫印迹的表达模式。由于据报道，在皮质脊髓束中表达的cDNAM，我们也研究了cDNAM表达的人肌萎缩侧索硬化症（ALS）的情况下，使用免疫组织化学。在大鼠脑组织中，生后早期在大脑皮质、基底节、海马和皮质脊髓束表达。随着出生后发育的进展，CALM表达仅限于大脑皮层第V层和第VI层的大锥体神经元和海马CA 1深层的锥体细胞。即使在相同的区域内，NRM阳性和阴性神经元也以马赛克模式分布。在肾脏中，在出生后早期，近端小管的上皮细胞和髓质的间充质细胞中表达CIMM;然而，随着髓质的快速生长，间充质细胞数量减少，髓质中的CIMM表达丢失。在人类ALS大脑中，我们观察到皮质脊髓束中的CIMM明显丢失，尤其是远端。我们的研究结果表明，在出生后的早期阶段，CARM可能在皮质和海马锥体细胞的发育中发挥作用，并在后期，在选定的神经元群体中执行细胞特异性功能。在肾脏中，CNOM可能在肾功能成熟中发挥作用。在这些细胞和器官中，CNOM的表达模式可能反映了其与T3或酮亚胺相互作用的意义。结果还表明CRYM可用作皮质脊髓束轴突变性的标志物。
The protein μ‐crystallin (CRYM) is a novel component of the marsupial lens that has two functions: it is a key regulator of thyroid hormone transportation and a reductase of sulfur‐containing cyclic ketimines. In this study, we examined changes of the expression pattern of CRYM in different rat organs during development using immunohistochemistry and immunoblotting. As CRYM is reportedly expressed in the corticospinal tract, we also investigated CRYM expression in human cases of amyotrophic lateral sclerosis (ALS) using immunohistochemistry. In the rat brain, CRYM was expressed in the cerebral cortex, basal ganglia, hippocampus and corticospinal tract in the early postnatal period. As postnatal development progressed, CRYM expression was restricted to large pyramidal neurons in layers V and VI of the cerebral cortex and pyramidal cells in the deep layer of CA1 in the hippocampus. Even within the same regions, CRYM‐positive and negative neurons were distributed in a mosaic pattern. In the kidney, CRYM was expressed in epithelial cells of the proximal tubule and mesenchymal cells of the medulla in the early postnatal period; however, CRYM expression in the medulla was lost as mesenchymal cell numbers decreased with the rapid growth of the medulla. In human ALS brains, we observed marked loss of CRYM in the corticospinal tract, especially distally. Our results suggest that CRYM may play roles in development of cortical and hippocampal pyramidal cells in the early postnatal period, and in the later period, performs cell‐specific functions in selected neuronal populations. In the kidney, CRYM may play roles in maturation of renal function. The expression patterns of CRYM may reflect significance of its interactions with T3 or ketimines in these cells and organs. The results also indicate that CRYM may be used as a marker of axonal degeneration in the corticospinal tract.
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影响因子：
9.8
作者：
Abe, S;Katagiri, T;Nakamura, Y
通讯作者：
Nakamura, Y
与 NADPH 复合的人微晶状体蛋白的晶体结构。
DOI：
--
发表时间：
2007
期刊：
Protein science : a publication of the Protein Society
影响因子：
--
作者：
Zhongjun Cheng;Lihua（孙丽华） Sun;Jianhua He;W. Gong
通讯作者：
W. Gong
影响因子：
4.4
作者：
Hallen, Andre;Cooper, Arthur J. L.;Karuso, Peter
通讯作者：
Karuso, Peter
影响因子：
9.8
作者：
Sapp, PC;Hosler, BA;Brown, RH
通讯作者：
Brown, RH
影响因子：
4.6
作者：
Brochier, Camille;Gaillard, Marie-Claude;de Chaldee, Michel
通讯作者：
de Chaldee, Michel