GENERATION OF LOBULOALVEOLAR DEVELOPMENT FROM ISOLATED RAT MAMMARY DUCTS AND END BUDS
GENERATION OF LOBULOALVEOLAR DEVELOPMENT FROM ISOLATED RAT MAMMARY DUCTS AND END BUDS
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DOI：
10.1177/39.9.1918944
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发表时间：
1991-09-01
影响因子：
3.2
通讯作者：
RUDLAND, PS
中科院分区：
文献类型：
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作者：
RUDLAND, PS
将Wistar-Furth大鼠原始乳腺切除的无芽导管断片、末端芽(TEB)或肺泡芽(ABS)植入同基因雌性大鼠肩关节间脂肪垫内，16周后可形成完整的导管突起，包括TEBS和ABS。用奋乃静处理受体大鼠1天或在12周后交配，然后在16周后分离得到的突起比未处理的宿主产生的突起大得多。突起分别由扩张的导管和小叶或扩张的导管和肺泡组成。用描绘上皮细胞、肌上皮细胞和乳泡细胞的试剂(单独花生凝集素，鼠酪蛋白的单抗和多克隆抗体)对突起进行组织化学和免疫细胞化学染色表明，所有突起的细胞组成和排列相似，无论其来源如何；这些突起也类似于奋乃静刺激或哺乳宿主的乳腺。有一个主要区别：ABS和TEBS产生的副产物的花生凝集素和抗酪蛋白的染色程度比导管产生的要大。在宿主停止哺乳后放置1年的导管植入物仍然被花生凝集素和抗酪蛋白，特别是管腔分泌物明显染色。因此，在无芽导管中存在着生成乳腺的完整的形态发生和细胞分化能力，但这种能力可以在TEBS/ABS中增强，或者在异位部位异常表达。
Implantation of excised bud-free ductal fragments (DUCTS), terminal end buds (TEBs), or alveolar buds (ABs) from virgin mammary glands of Wistar-Furth rats into interscapular fat pads of syngeneic female rats produces, after 16 weeks, complete ductal outgrowths including TEBs and ABs. Treatment of the recipient rats with perphenazine for 1 day or mating them after 12 weeks and then isolating the resultant outgrowths after 16 weeks produces significantly larger outgrowths than those from untreated hosts. The outgrowths consist of distended ducts and lobules or distended ducts and alveoli, respectively. Histochemical and immunocytochemical staining of the outgrowths with reagents that depict epithelial, myoepithelial, and lactating alveolar cells (peanut lectin alone, monoclonal and polyclonal antibodies to rat caseins) indicate similar cell compositions and arrangements for all outgrowths irrespective of their source; these are also similar to the mammary glands of the perphenazine-stimulated or lactating hosts. There is one major difference: the degree of staining of peanut lectin alone and the anti-caseins is greater for outgrowths produced by the ABs and TEBs than for those produced by the DUCTs. DUCT implants left for 1 year after cessation of lactation of the hosts are still stained appreciably by peanut lectin alone and by the anti-caseins, particularly the luminal secretions. Therefore, the complete morphogenetic and cell differentiating ability for generating mammary glands is present in bud-free ducts, but this ability can be enhanced in TEBs/ABs or abnormally expressed at ectopic sites.