Genomic characterization and application of a novel bacteriophage STG2 capable of reducing planktonic and biofilm cells of Salmonella
Genomic characterization and application of a novel bacteriophage STG2 capable of reducing planktonic and biofilm cells of Salmonella
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DOI：
10.1016/j.ijfoodmicro.2022.109999
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发表时间：
2022-11-03
影响因子：
5.4
通讯作者：
Miyamoto, Takahisa
中科院分区：
文献类型：
--
作者：
Duc, Hoang Minh;Zhang, Yu;Miyamoto, Takahisa
作为一种主要的食源性病原体，沙门氏菌可在全球范围内引起严重的食物中毒暴发。噬菌体疗法越来越被认为是生物防治食源性致病菌的最有前途的抗菌剂之一。在本研究中，鉴定了一种能够感染肠炎沙门氏菌和鼠伤寒沙门氏菌的裂解噬菌体STG2，并评价了其在甘蓝和不同表面上减少这些浮游和生物膜形式的食源性病原体的效果。基因组特征分析表明，STG2噬菌体为噬菌体属噬菌体，其dsDNA基因组长114,275bp，不含任何与抗生素耐药性、毒素、溶原性或毒力因子相关的基因。此外，噬菌体STG2在减少卷心菜上的浮游细胞以及在卷心菜、聚苯乙烯和不锈钢上形成的生物膜方面表现出很好的效果，表明噬菌体STG2能够同时控制食品和食品相关表面的肠炎沙门氏菌和鼠伤寒沙门氏菌污染。
As one major foodborne pathogen, Salmonella can cause serious food poisoning outbreaks worldwide. Bacteriophage therapy is increasingly considered as one of the promising antibacterial agents for the biocontrol of foodborne pathogens. In the current study, a lytic phage STG2 capable of infecting S. enteritidis and S. typhimurium was characterized, and its efficacy in reducing these foodborne pathogens in both planktonic and biofilm forms was evaluated on cabbage and various surfaces. Genomic characterization revealed that phage STG2 was Siphoviridae phage (Epseptimavirus genus) with a dsDNA genome comprising of 114,275 bp and its genome does not contain any genes associated to antibiotic resistance, toxins, lysogeny, or virulence factors. Additionally, phage STG2 exhibited great efficacy in reducing (>2 Log) planktonic cells on cabbage as well as the biofilms formed on cabbage, polystyrene, and stainless steel, suggesting that phage STG2 is capable of simultaneously controlling both S. enteritidis and S. typhimurium contaminations on food and food-related surfaces.