Protective Roles of Xijiao Dihuang Tang on Coronary Artery Injury in Kawasaki Disease
Protective Roles of Xijiao Dihuang Tang on Coronary Artery Injury in Kawasaki Disease
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DOI：
10.1007/s10557-021-07277-w
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发表时间：
2021-10-19
影响因子：
3.4
通讯作者：
Jia, Chang
中科院分区：
文献类型：
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作者：
Zhang, Jian;Zhuge, Yingzhi;Jia, Chang
目的犀角地黄汤（XJDHT）是由水牛角（Cornu Bubali）、党参（Gaertn.）华盛顿，乳花海棠，和牡丹安德鲁斯。最早见于唐代孙思邈所著的医学经典《北极千金要方》。具有很强的解热、抗凝作用，临床上用于治疗各种失血、紫黑斑、中暑、舌炎等。川崎是一种以全身性血管炎为主要病变的小儿急性发热性疾病。方法采用白念珠菌水溶性成分（CAWS）诱导的小鼠冠状动脉炎模型和用肿瘤坏死因子（TNF-α）诱导的KD细胞模型，观察XJDHT对KD的保护作用。采用α诱导法，观察消积散痛汤对川崎病冠状动脉损伤的影响，并探讨其作用机制小鼠，如炎症减少和促炎细胞因子白细胞介素（IL）-1 β和TNF-α表达下调所证明。体外研究表明，消积散痛汤能促进细胞增殖，抑制细胞凋亡，改善线粒体功能。后续研究表明，消积散痛汤通过PI 3 K/Akt-NF kappa B信号通路对内皮细胞损伤具有保护作用。结论XJDHT通过PI 3 K/Akt-NF kappa B信号通路抑制KD大鼠冠状动脉内皮细胞损伤，减轻KD大鼠冠状动脉损伤。
Purpose Xijiao Dihuang Tang (XJDHT) is a classical formula of traditional Chinese medicine constituted of Cornu Bubali, Rehmannia glutinosa (Gaertn.) DC., Paeonia lactiflora Pall., and Paeonia suffruticosa Andrews. It was first mentioned in the medical classic "Beiji Qianjin Yaofang" written by Simiao Sun in Tang Dynasty. It shows very strong antipyretic and anticoagulant effects and has been clinically applied to treat various type of blood loss, purple and black spots, heat stroke, and glossitis. Kawasaki disease (KD) is considered as a kind of acute febrile illness in children with systemic vasculitis as the main lesions. The aim of this research is to clarify whether XJDHT can play a protective role in KD.Methods A mouse model of Candida albicans water-soluble fraction (CAWS)-induced coronary arteritis and a KD cell model with tumor necrosis factor (TNF)-alpha induction were employed to investigate the potential effect and mechanism of XJDHT on coronary artery injury in KD.Results Data showed that XJDHT remarkably alleviated the coronary artery injury of KD mice, as evidenced by reduced inflammation and downregulated expression of pro-inflammatory cytokines interleukin (IL)-1 beta and TNF-alpha. In vitro investigation showed that XJDHT could promote cell proliferation, inhibit cell apoptosis, and improve mitochondrial functions. Subsequent studies demonstrated that XJDHT rescued endothelial cell injury by PI3K/Akt-NF kappa B signaling pathway. Component analysis of XJDHT detected thirty-eight chemically active ingredients, including paeoniflorin, albiflorin, and paeoniflorigenone, which in in vitro experiments exhibited significant rescue effects on TNF-alpha-mediated endothelial cell injury.Conclusion Our findings demonstrated that XJDHT mitigated coronary artery injury of KD through suppressing endothelial cell damage via PI3K/Akt-NF kappa B signaling.