A porous silicon optical biosensor: Detection of reversible binding of IgG to a protein A-modified surface
A porous silicon optical biosensor: Detection of reversible binding of IgG to a protein A-modified surface
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DOI：
10.1021/ja991421n
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发表时间：
1999-09-01
影响因子：
15
通讯作者：
Sailor, MJ
中科院分区：
文献类型：
--
作者：
Dancil, KPS;Greiner, DP;Sailor, MJ
的可逆性，特异性，稳定性和缩放的信号响应分析物质量进行了定量的多孔硅基光学干涉生物传感器。所研究的传感器系统由蛋白A修饰的多孔硅薄层（5 μ m）组成。用水性人IgG分析物的各种片段探测系统。该传感器通过测量多孔硅层反射白色光的法布里-珀罗条纹来工作。分子结合被检测为这些条纹的波长的偏移。通过改变流动池中的溶液pH，将IgG添加到蛋白A修饰的表面和从蛋白A修饰的表面去除，并且发现该系统通过几个开关循环是可逆的。用于将蛋白A连接到多孔Si表面的分子并入牛血清白蛋白（BSA）。发现这种方法完全消除了由于非特异性结合引起的信号，通过将传感器暴露于IgG的F（ab '）（2）片段（其不结合蛋白A）来测试。还发现接头/蛋白A修饰的表面在所用的水性缓冲溶液中对氧化是稳定的。发现法布里-珀罗条纹的位移与多孔Si层中结合的分析物的质量成比例。
The reversibility, specificity, stability, and scaling of signal response to analyte mass were quantified for a porous silicon-based optical interferometric biosensor. The sensor system studied consisted of a thin layer (5 mu m) of porous silicon modified with Protein A. The system was probed with various fragments of an aqueous Human IgG analyte. The sensor operates by measurement of the Fabry-Perot fringes in the white light reflection spectrum fi om the porous silicon layer. Molecular binding is detected as a shift in wavelength of these fringes. IgG was added to and removed from the protein A-modified surface by changing solution pH in a flow cell, and the system was found to be reversible through several on-off cycles. The molecule used to link protein A to the porous Si surface incorporated bovine serum albumin (BSA). This approach was found to completely eliminate signal due to nonspecific binding, tested by exposure of the sensor to the F(ab')(2) fragment of IgG (which does not bind to protein A). The linker/protein A-modified surface was also found to be stable toward oxidation in the aqueous buffer solutions used. The shift in the Fabry-Perot fringes was found to scale with the mass of analyte bound in the porous Si layer.