Influence of TRPV3 mutation on hair growth cycle in mice
Influence of TRPV3 mutation on hair growth cycle in mice
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DOI：
10.1016/j.bbrc.2007.08.170
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发表时间：
2007-11-23
影响因子：
3.1
通讯作者：
Sakata, Tsuneaki
中科院分区：
文献类型：
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作者：
Imura, Kinichi;Yoshioka, Takeshi;Sakata, Tsuneaki
我们最近报道，Gly 573 Ser取代的瞬时受体电位阳离子通道，亚家族V成员3（TRPV 3）引起的DS-Nh小鼠脱发。为了进一步阐明这种突变对自发无毛表型发展的影响，我们研究了DS-Nh和DS小鼠对表皮片的温度反应。结果表明，该突变为功能获得性突变。我们还对两种品系的皮肤进行了遗传学和组织学分析。DNA微阵列数据显示，与生长期相关的角蛋白相关蛋白16-1、16-3和16-9基因的水平在DS-Nh小鼠的皮肤中比三天大的DS小鼠的皮肤中降低。组织学分析表明，生长期持续在DS-Nh小鼠，并在DS中看到休止期，而不是DS-Nh小鼠在21日龄。TRPV 3的调节似乎对啮齿动物的适当毛发发育很重要。(C)2007爱思唯尔公司All rights reserved.
We recently reported that Gly573Ser substitution of the transient receptor potential cation channel, subfamily V member 3 (TRPV3) caused hair loss in DS-Nh mice. To further elucidate the effects of this mutation on the development of the spontaneous hairless phenotype, we examined the temperature-response to epidermal sheets from DS-Nh and DS mice. It was indicated that the mutation was gain-of-function. We also performed genetic and histological analyses with both strain skins. DNA microarray data revealed that the levels of keratin-associated protein 16-1, 16-3, and 16-9 genes related to the anagen phase were decreased in the skins of DS-Nh mice compared with those of three days old DS mice. Histological analysis revealed that the anagen phase persisted in DS-Nh mice, and that the telogen phase was seen in DS but not DS-Nh mice at 21 days of age. Regulation of TRPV3 appears to be important for appropriate hair development in rodents. (C) 2007 Elsevier Inc. All rights reserved.