The interfascicular trigeminal nucleus: A precerebellar nucleus in the mouse defined by retrograde neuronal tracing and genetic fate mapping
The interfascicular trigeminal nucleus: A precerebellar nucleus in the mouse defined by retrograde neuronal tracing and genetic fate mapping
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DOI：
10.1002/cne.23200
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发表时间：
2013-02-15
影响因子：
2.5
通讯作者：
Watson, Charles
中科院分区：
文献类型：
--
作者：
Fu, Yuhong;Tvrdik, Petr;Watson, Charles
我们在小鼠三叉神经运动根的新兴纤维中发现了一个以前未报道的小脑前核，我们称之为三叉神经束间核（IF5）。该核先前在啮齿动物脑图谱中被命名为三叉神经运动核（5TT）的鼓张肌部分，因为它被认为是三叉神经运动核的小运动神经元的子集，支配鼓张肌。然而，在小脑中注射逆行示踪剂后，发现IF5中的标记神经元是胆碱乙酰转移酶（ChAT）阴性的，表明它们不是运动神经元。IF5的细胞在Wnt1Cre和Atoh1 CreER谱系命运映射的小鼠中被强烈标记，与从菱形唇产生并发出苔藓纤维的主要小脑前核共同。从小鼠Hoxa3，Hoxb1，和Egr 2 Cre标记的谱系的部分分析表明，IF5的神经元产生从rhombomeres尾部到rhombomeres 4，最有可能从rhombomeres 68。我们的结论是，IF5是一个显着的小脑前核在小鼠中的苔藓纤维小脑前核，从尾侧菱形唇出现的共享发育基因表达特征。神经学比较杂志521：697708，2013. (c)2012 Wiley Periodicals，Inc.
We have found a previously unreported precerebellar nucleus located among the emerging fibers of the motor root of the trigeminal nerve in the mouse, which we have called the interfascicular trigeminal nucleus (IF5). This nucleus had previously been named the tensor tympani part of the motor trigeminal nucleus (5TT) in rodent brain atlases, because it was thought to be a subset of small motor neurons of the motor trigeminal nucleus innervating the tensor tympani muscle. However, following injection of retrograde tracer in the cerebellum, the labeled neurons in IF5 were found to be choline acetyltransferase (ChAT) negative, indicating that they are not motor neurons. The cells of IF5 are strongly labeled in mice from Wnt1Cre and Atoh1 CreER lineage fate mapping, in common with the major precerebellar nuclei that arise from the rhombic lip and that issue mossy fibers. Analysis of sections from mouse Hoxa3, Hoxb1, and Egr2 Cre labeled lineages shows that the neurons of IF5 arise from rhombomeres caudal to rhombomere 4, most likely from rhombomeres 68. We conclude that IF5 is a significant precerebellar nucleus in the mouse that shares developmental gene expression characteristics with mossy fiber precerebellar nuclei that arise from the caudal rhombic lip. J. Comp. Neurol. 521:697708, 2013. (c) 2012 Wiley Periodicals, Inc.