Determining iron oxide nanoparticle heating efficiency and elucidating local nanoparticle temperature for application in agarose gel-based tumor model.
Determining iron oxide nanoparticle heating efficiency and elucidating local nanoparticle temperature for application in agarose gel-based tumor model.
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DOI：
10.1016/j.msec.2016.05.086
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发表时间：
2016-11-01
期刊：
影响因子：
--
通讯作者：
Brazel CS
中科院分区：
文献类型：
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作者：
Shah RR;Dombrowsky AR;Paulson AL;Johnson MP;Nikles DE;Brazel CS
磁性氧化铁纳米颗粒（MNP）已被开发用于磁流体热疗（MFH）癌症治疗，其中癌细胞通过施加高频磁场产生的热量来治疗。这种热量也被认为是触发化疗药物释放的机制。在这些情况下，具有最佳加热性能的MNP可用于最大化治疗效果，同时最小化MNP的所需剂量。在这项研究中，加热效率（或特定的吸收率，SAR）的两种类型的MNP的实验评估，然后从它们的磁性预测。MNP也被纳入聚（乙二醇-B-己内酯）胶束的核心，共定位与罗丹明B荧光染料连接到聚己内酯监测局部，在磁加热过程中的纳米级温度。尽管这些MNP产生的SAR相对较高，但通过磁性胶束核心中的荧光测量，没有显著的温度升高超过在本体溶液中观察到的温度。还将MNP掺入到大规模琼脂糖凝胶系统中，该系统模拟由MNP靶向并被健康组织包围的肿瘤。基于琼脂糖的肿瘤模型表明，靶向MNP可以在足够的MNP浓度下达到肿瘤内部的高热温度，同时在肿瘤周围的健康组织中引起最小的温度升高。
Magnetic iron oxide nanoparticles (MNPs) have been developed for magnetic fluid hyperthermia (MFH) cancer therapy, where cancer cells are treated through the heat generated by application of a high frequency magnetic field. This heat has also been proposed as a mechanism to trigger release of chemotherapy agents. In each of these cases, MNPs with optimal heating performance can be used to maximize therapeutic effect while minimizing the required dosage of MNPs. In this study, the heating efficiencies (or specific absorption rate, SAR) of two types of MNPs were evaluated experimentally and then predicted from their magnetic properties. MNPs were also incorporated in the core of poly(ethylene glycol-b-caprolactone) micelles, co-localized with rhodamine B fluorescent dye attached to polycaprolactone to monitor local, nanoscale temperatures during magnetic heating. Despite a relatively high SAR produced by these MNPs, no significant temperature rise beyond that observed in the bulk solution was measured by fluorescence in the core of the magnetic micelles. MNPs were also incorporated into a macro-scale agarose gel system that mimicked a tumor targeted by MNPs and surrounded by healthy tissues. The agarose-based tumor models showed that targeted MNPs can reach hyperthermia temperatures inside a tumor with a sufficient MNP concentration, while causing minimal temperature rise in the healthy tissue surrounding the tumor.