Inhibitory and excitatory perigenital-to-bladder spinal reflexes in the cat
Inhibitory and excitatory perigenital-to-bladder spinal reflexes in the cat
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DOI：
10.1152/ajprenal.00443.2007
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发表时间：
2008-03-01
影响因子：
4.2
通讯作者：
de Groat, William C.
中科院分区：
文献类型：
--
作者：
Tai, Changfeng;Shen, Bing;de Groat, William C.
这项研究表明，在清醒的慢性脊髓损伤（SCI）猫反射生殖器周围的皮肤区域膀胱可以是抑制性或兴奋性。电性生殖器周围刺激频率在5和7 Hz之间显着抑制大幅度节律反射膀胱活动，而频率在20和40 Hz之间诱导大幅度膀胱收缩，即使在低膀胱容量时，反射膀胱活动不存在。随着刺激强度的增加（5-30 V，0.2-ms脉冲宽度），抑制和兴奋效应均增强。在膀胱测压期间，抑制性刺激（7 Hz）使排尿量阈值显著增加，比对照量高35 +/- 13%，而兴奋性刺激（30 Hz）使阈值显著降低21 +/-3%。用棉签反复轻轻抚摸生殖器周围皮肤施加的机械性生殖器周围刺激仅对膀胱产生兴奋作用。电刺激和机械刺激都能引起大幅度（> 30 cm H（2）O）膀胱收缩，这种收缩在一定范围的膀胱容量（容量的10-90%）内相对一致。然而，兴奋性电刺激仅诱导平均持续42.2 +/-3.9秒的膀胱收缩，但机械刺激诱导的膀胱收缩持续与刺激持续的时间一样长（2-3分钟）。兴奋性电刺激或机械性生殖器周围刺激也引起刺激后排尿。通过非侵入性方法抑制或刺激膀胱的能力可以显著改变目前SCI后膀胱功能的临床管理。
This study revealed that in awake chronic spinal cord-injured (SCI) cats reflexes from perigenital skin area to the bladder can be either inhibitory or excitatory. Electrical perigenital stimulation at frequencies between 5 and 7 Hz significantly inhibited large-amplitude rhythmic reflex bladder activity, whereas frequencies between 20 and 40 Hz induced large-amplitude bladder contractions even at low bladder volumes when reflex bladder activity was absent. Both inhibitory and excitatory effects were enhanced as the stimulation intensity increased (5-30 V, 0.2-ms pulse width). During cystometrograms, the inhibitory stimulation (7 Hz) significantly increased the micturition volume threshold 35 +/- 13% above the control volume, while the excitatory stimulation (30 Hz) significantly reduced the threshold 21 +/- 3%. Mechanical perigenital stimulation applied by repeated light stroking of the perigenital skin with a cotton swab only induced an excitatory effect on the bladder. Both electrical and mechanical perigenital stimuli induced large-amplitude (> 30 cm H(2)O) bladder contractions that were relatively consistent over a range of bladder volumes (10-90% of the capacity). However, the excitatory electrical stimulation only induced bladder contractions lasting on average 42.2 +/- 3.9 s, but the mechanical stimulation induced bladder contractions that lasted as long as the stimulation continued (2-3 min). Excitatory electrical or mechanical perigenital stimulation also induced poststimulus voiding. The ability to either inhibit or excite the bladder by noninvasive methods could significantly transform the current clinical management of bladder function after SCI.