Genetic evaluation of childhood acute lymphoblastic leukemia in Iraq using FTA cards
Genetic evaluation of childhood acute lymphoblastic leukemia in Iraq using FTA cards
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DOI：
10.1002/pbc.24055
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发表时间：
2012-09-01
影响因子：
3.2
通讯作者：
Koike, Kenichi
中科院分区：
文献类型：
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作者：
Al-Kzayer, Lika'a Fasih Y.;Sakashita, Kazuo;Koike, Kenichi
背景在伊拉克还没有儿童白血病的基因检查。我们在这里报告TEL-AML 1，E2 A-PBX 1，MLL-AF 4和BCR-ABL嵌合体转录本的频率在264名新诊断为急性淋巴细胞白血病（ALL）的伊拉克儿童，使用FTA卡浸渍骨髓穿刺液或全血。患者和方法根据标准的法国-美国-英国形态学标准诊断ALL。根据储存温度和时间的结果，大多数FTA样本在伊拉克五家医院中在4摄氏度下保存长达6周，然后转移到日本进行分子分析。采用巢式逆转录-聚合酶链反应（Nested RT-PCR）进行分析。结果264例ALL患者中32例（12.1%）检测到TEL-AML 1嵌合体转录产物。分别有11例（4.2%）患者、4例（1.5%）患者和11例（4.2%）患者存在E2 A-PBX 1 mRNA、MLL-AF 4 mRNA和BCR-ABL mRNA。1例患者同时具有TEL-AML 1和E2 A-PBX 1融合基因。伊拉克ALL儿童中TEL-AML 1的发病率似乎与约旦（12%）和科威特（7%）相似或略高。E2 A-PBX 1或BCR-ABL ALL患者的患病率和临床结果与其他地方报告的数据相当。结论通过FTA卡开展国际合作有助于提高低收入和欠发达国家恶性血液病患者的诊断和管理水平。儿科血液癌症2012;59：461467。(c)2012 Wiley Periodicals，Inc.
Background Genetic examination of childhood leukemia has not been available in Iraq. We here report the frequency of TEL-AML1, E2A-PBX1, MLL-AF4, and BCR-ABL chimeric transcripts in 264 Iraqi children newly diagnosed with acute lymphoblastic leukemia (ALL), using FTA cards impregnated with bone marrow aspirate or whole blood. Patients and methods The diagnosis of ALL was made according to standard French-American-British morphologic criteria. Based on the results of storage temperature and duration, most of the FTA samples were preserved at 4 degrees C for up to 6 weeks in five Iraqi hospitals and then transferred to Japan for molecular analysis. Nested reverse transcription-polymerase chain reaction was adopted for the analysis. Results TEL-AML1 chimeric transcript product was found in 32 (12.1%) of 264 ALL patients. Eleven (4.2%) patients, 4 (1.5%) patients, and 11 (4.2%) patients had E2A-PBX1 mRNA, MLL-AF4 mRNA, and BCR-ABL mRNA, respectively. One patient had both TEL-AML1 and E2A-PBX1 fusion genes. The incidence of TEL-AML1 in Iraqi ALL children appears to be similar to or slightly higher than those of Jordan (12%) and Kuwait (7%). The prevalence and clinical findings of ALL patients with either E2A-PBX1 or BCR-ABL were comparable to the data reported elsewhere. Conclusion International collaboration via FTA cards may be helpful to improve diagnosis and management of patients with hematological malignancies in low-income and underdeveloped countries. Pediatr Blood Cancer 2012;59:461467. (c) 2012 Wiley Periodicals, Inc.