Age-dependent regulation of lipogenesis in human and rat adipocytes
Age-dependent regulation of lipogenesis in human and rat adipocytes
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DOI：
10.1210/jc.2003-030994
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发表时间：
2004-09-01
影响因子：
5.8
通讯作者：
Marcus, C
中科院分区：
文献类型：
--
作者：
Kamel, AF;Norgren, S;Marcus, C
脂肪细胞代谢的调节对于脂肪组织生长以及因此对于肥胖症的发展是重要的。本研究旨在探讨基础和胰岛素诱导的脂肪生成，葡萄糖转运和葡萄糖转运蛋白的表达在人类和大鼠脂肪细胞从不同年龄组的调节，该研究包括21名婴儿，21名儿童，9名成年人，80名雄性断奶和20名雄性成年Fischer大鼠。脂肪生成实验在葡萄糖转运是速率限制的条件下进行。婴儿和儿童的基础脂肪生成大约是成人的3倍，而婴儿胰岛素诱导的脂肪生成是儿童和成人的2倍。在大鼠中，基础脂肪生成，胰岛素诱导的脂肪生成和胰岛素敏感性是2倍以上的断奶比成年动物。此外，基础和胰岛素诱导的葡萄糖转运是两倍以上的断奶比成年大鼠。在人或大鼠脂肪细胞中的任何年龄组之间的GLUT1或GLUT4含量均未检测到差异。总之，在生命早期脂肪细胞中基础和胰岛素刺激的脂肪生成增加。这可能会促进早期脂肪组织的生长。这些数据表明，基础和胰岛素刺激的脂肪生成的年龄依赖性变化受到不同的调节。
The regulation of adipocyte metabolism is of importance for adipose tissue growth and therefore also for the development of obesity. This study was designed to investigate the regulation of basal and insulin-induced lipogenesis, glucose transport, and glucose transporter protein expression in human and rat adipocytes from different age groups.The study included 21 infants, 21 children, nine adults, and 80 male weaned and 20 male adult Fischer rats. The lipogenesis experiments were performed under conditions at which glucose transport is rate limiting. Basal lipogenesis was approximately three times higher in infants and children than in adults, whereas insulin-induced lipogenesis was two times higher in infants than in children and adults. In rats, basal lipogenesis, insulin-induced lipogenesis, and insulin sensitivity were two times higher in weaned than in adult animals. Moreover, basal and insulin-induced glucose transport were two times higher in weaned than in adult rats. No differences were detected in GLUT1 or GLUT4 content between any of the age groups in human or in rat adipocytes.In conclusion, basal and insulin-stimulated lipogenesis are increased in adipocytes early in life. This may promote adipose tissue growth in early age. The data indicate that age-dependent variation in basal and insulin-stimulated lipogenesis is differently regulated.