The thermo-regulated genetic switch of deep-sea filamentous phage SW1 and its distribution in the Pacific Ocean
The thermo-regulated genetic switch of deep-sea filamentous phage SW1 and its distribution in the Pacific Ocean
复制标题
深海丝状噬菌体SW1的温度调节遗传开关及其在太平洋的分布
DOI：
10.1093/femsle/fnaa094
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发表时间：
2020
影响因子：
2.1
通讯作者：
Jian Huahua
中科院分区：
文献类型：
--
作者：
Meng Canxing;Li Site;Fan Qilian;Chen Rouke;Hu Yang;Xiao Xiang;Jian Huahua
病毒特别是噬菌体在深海生态系统中具有重要作用，但其对环境变化的响应机制尚不清楚。我们以前的工作特点是冷活性丝状噬菌体SW 1感染深海细菌Shewanella piezotoleransWP 3;然而，潜在的机制，假定的热调节的遗传开关的SW 1仍然不清楚。在这项研究中，DNA拷贝数和mRNA丰度的深海噬菌体SW 1的定量在其宿主的整个生命周期。piezotoleransWP 3在不同温度下。我们的结果表明，SW 1的诱导依赖于阈值温度（4°C），但这种依赖性与温度梯度不成比例。RNA-Seq分析揭示了两个在4°C下高度转录的区域，并验证了SW 1基因组中存在长的3′非翻译区（UTR）。募集分析显示，SW 1样丝状病毒在深海（水深>1000 m）和光上层和中层（水深<1000 m）中普遍存在，提示SW 1中揭示的温度调节遗传开关可能在海洋中广泛分布。
Viruses, especially bacteriophages, are thought to have important functions in the deep-sea ecosystem, but little is known about the induction mechanism of benthic phages in response to environmental change. Our prior work characterized a cold-active filamentous phage SW1 that infects the deep-sea bacteriumShewanella piezotoleransWP3; however, the underlying mechanism of the putative thermo-regulated genetic switch of SW1 is still unclear. In this study, the DNA copy number and mRNA abundance of the deep-sea phage SW1 were quantified in the whole life cycle of its hostS. piezotoleransWP3 at different temperatures. Our results demonstrated that the induction of SW1 is dependent on a threshold temperature (4°C), but this dependency is not proportional to temperature gradient. RNA-Seq analyses revealed two highly transcribed regions at 4°C and verified the presence of a long 3′ untranslated region (UTR) in the SW1 genome. Interestingly, recruitment analysis showed that SW1-like inoviruses prevail in deep sea (depth >1000 m) and photic epipelagic and mesopelagic zones (depth <1000 m), which suggested that the thermo-regulated genetic switch revealed in SW1 may be widely distributed in the ocean.