The potential of chondrogenic pre-differentiation of adipose-derived mesenchymal stem cells for regeneration in harsh nucleus pulposus microenvironment
The potential of chondrogenic pre-differentiation of adipose-derived mesenchymal stem cells for regeneration in harsh nucleus pulposus microenvironment
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脂肪间充质干细胞的软骨预分化在恶劣的髓核微环境中再生的潜力
DOI：
10.1177/1535370216662362
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发表时间：
2016
影响因子：
3.2
通讯作者：
Chen Qi-xin
中科院分区：
文献类型：
--
作者：
Wang Jingkai;Tao Yiqing;Zhou Xiaopeng;Li Hao;Liang Chengzhen;Li Fangcai;Chen Qi-xin
近年来的研究表明，以细胞为基础的治疗可能是一种很有前途的方法来治疗椎间盘退变。尽管椎间盘内恶劣的微环境对植入细胞仍具有挑战性，但先前的文献表明，通过在移植前预处理移植细胞可以克服这一挑战。因此，我们设计了这项研究，以确定在椎间盘样微环境中，其特征在于有限的营养，酸性和高渗透性的体外软骨预分化对脂肪来源的间充质干细胞的潜在影响。将大鼠脂肪间充质干细胞分为5组，包埋于II型胶原支架中，分别在软骨分化培养基中培养0、3、7、10和14 d。然后，将脂肪间充质干细胞植入椎间盘并在椎间盘样条件下培养。采用kit-8细胞计数法、实时荧光定量聚合酶链反应、Western blotting和免疫荧光法检测脂肪间充质干细胞的增殖和分化情况。通过在椎间盘样条件下的第一周分析，结果显示预分化7和10天的脂肪来源的间充质干细胞的增殖能力和细胞外基质合成能力比对照相对更高。结论：大鼠脂肪间充质干细胞在成软骨培养基中预分化7 ~ 10 d，可促进脂肪间充质干细胞在椎间盘样条件下的再生效果，预分化细胞有望成为椎间盘再生医学的细胞来源。
Recent studies indicated that cell-based therapy could be a promising approach to treat intervertebral disc degeneration. Though the harsh microenvironment in disc is still challenging to implanted cells, it could be overcome by pre-conditioning graft cells before transplantation, suggested by previous literatures. Therefore, we designed this study to identify the potential effect of chondrogenic pre-differentiation on adipose-derived mesenchymal stem cells in intervertebral disc-like microenvironment, characterized by limited nutrition, acidic, and high osmosis in vitro. Adipose-derived mesenchymal stem cells of rat were divided into five groups, embedded in type II collagen scaffold, and cultured in chondrogenic differentiation medium for 0, 3, 7, 10, and 14 days. Then, the adipose-derived mesenchymal stem cells were implanted and cultured in intervertebral disc-like condition. The proliferation and differentiation of adipose-derived mesenchymal stem cells were evaluated by cell counting kit-8 test, real-time quantitative polymerase chain reaction, and Western blotting and immunofluorescence analysis. Analyzed by the first week in intervertebral disc-like condition, the results showed relatively greater proliferative capability and extracellular matrix synthesis ability of the adipose-derived mesenchymal stem cells pre-differentiated for 7 and 10 days than the control. We concluded that pre-differentiation of rat adipose-derived mesenchymal stem cells in chondrogenic culture medium for 7 to 10 days could promote the regeneration effect of adipose-derived mesenchymal stem cells in intervertebral disc-like condition, and the pre-differentiated cells could be a promising cell source for disc regeneration medicine.