Effects of renal denervation on endogenous ouabain in spontaneously hypertensive rats.
Effects of renal denervation on endogenous ouabain in spontaneously hypertensive rats.
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DOI：
10.1590/acb371102
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发表时间：
2023
影响因子：
1.1
通讯作者：
Li, Jianling
中科院分区：
文献类型：
--
作者：
Lai, Xiaomei;Wen, Hong;Yang, Tingting;Qin, Fei;Zhong, Xiaoge;Pan, Yajin;Yu, Jie;Huang, Jing;Li, Jianling
目的 研究肾去神经支配 (RDN) 对自发性高血压大鼠 (SHR) 内源性哇巴因 (EO) 分泌的作用。 16只12周龄雄性SHR随机分为去肾神经组（RDNX组）和假手术组（sham组），8只年龄匹配的Wistar京都大鼠（WKY）作为对照组。评估了EO浓度、Na+-K+-ATP酶活性和Na+-K+-ATP酶的表达。假手术组血清、肾脏和下丘脑中的EO水平高于RDNX组（p < 0.05）。与假手术组相比，去神经手术后的肾Na+-K+-ATP酶活性显着降低（p < 0.05）。肾脏去甲肾上腺素（NE）含量与Na+-K+-ATP酶活性呈正相关（r2 = 0.579）。与假手术组相比，RDNX组肾Na+-K+-ATPase α1亚基mRNA表达量下调（P < 0.05），而各组间肾Na+-K+-ATPase α1亚基mRNA表达量差异无统计学意义（P = 0.63）。免疫组化分析显示，三组肾组织Na+-K+-ATP酶表达差异有统计学意义（P < 0.05）。这些实验表明，RDN 通过降低 SHR 中的 EO 水平、Na+-K+-ATPase 活性和肾脏 Na+-K+-ATPase α1 亚基表达而发挥抗高血压作用。
To investigate the role of renal denervation (RDN) on endogenous ouabain (EO) secretion in spontaneously hypertensive rats (SHR). Sixteen 12-week-old male SHR were randomly separated into the renal denervation group (RDNX group) and sham operation group (sham group), and eight age-matched Wistar Kyoto rats (WKY) were served as control group. EO concentrations, the Na+- K+-ATPaseactivity, and the expression of Na+-K+-ATPase were assessed. EO levels in serum, kidneys and hypothalamus of sham group were higher than in RDNX group (p < 0.05). Renal Na+-K+-ATPase activity subjected to denervation surgery showed significantly reduction when compared with the sham groups (p < 0.05). A positive correlation existed between norepinephrine (NE) content and Na+-K+-ATPase activity in the kidney (r2 = 0.579). Renal Na+-K+-ATPase α1 subunit mRNA expression was down-regulated in the RDNX group compared with the sham group (P < 0.05), while renal Na+-K+-ATPase α1 subunit mRNA expression was no statistical significance between the groups (P = 0.63). Immunohistochemical analysis showed that there were significant differences in the renal expression of Na+-K+-ATPasebetween the three groups (P < 0.05). These experiments demonstrate that RDN exerted an anti-hypertensive effect with reduction of EO levels and Na+-K+-ATPase activity and Na+-K+-ATPase α1 subunit expression of kidney in SHR.
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DOI：
10.1161/hypertensionaha.115.06415
发表时间：
2016-02
期刊：
Hypertension (Dallas, Tex. : 1979)
影响因子：
--
作者：
Lanzani C;Gatti G;Citterio L;Messaggio E;Delli Carpini S;Simonini M;Casamassima N;Zagato L;Brioni E;Hamlyn JM;Manunta P
通讯作者：
Manunta P
影响因子：
21.1
作者：
Bagrov AY;Shapiro JI;Fedorova OV
通讯作者：
Fedorova OV
影响因子：
4.9
作者：
Mancia, Giuseppe
通讯作者：
Mancia, Giuseppe
影响因子：
3.1
作者：
Ferrandi, M;Manunta, P;Bianchi, G
通讯作者：
Bianchi, G
影响因子：
8.3
作者：
Bigazzi, Roberto;Zagato, Laura;Manunta, Paolo
通讯作者：
Manunta, Paolo