Mitochondrial transplantation for myocardial protection in diabetic hearts
Mitochondrial transplantation for myocardial protection in diabetic hearts
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DOI：
10.1093/ejcts/ezz326
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发表时间：
2020-05-01
影响因子：
3.4
通讯作者：
McCully, James D.
中科院分区：
文献类型：
--
作者：
Doulamis, Ilias P.;Guariento, Alvise;McCully, James D.
目的：2 型糖尿病导致线粒体功能障碍，从而增加心肌对缺血再灌注损伤的易感性。我们研究了从糖尿病或非糖尿病供体分离的线粒体移植对糖尿病大鼠心脏免受热全面缺血和再灌注的心脏保护作用的功效。 方法：来自 Zucker 糖尿病脂肪 (ZDF fa/fa) 大鼠（每组 n = 6）的离体灌注心脏经历 30 分钟的热全面缺血和 120 分钟再灌注。在再灌注之前，将单独的载体（VEH）或含有从ZDF（MTZDF）或非糖尿病Zucker瘦（ZL +/？）（MTZL）骨骼肌中分离的线粒体的载体通过主动脉插管输送至冠状动脉。 结果：在30分钟的整体缺血和120分钟的再灌注后，与MTZDF和MTZL组相比，左心室发展压力显着增加至 VEH 组（MTZDF：92.85.2 mmHg vs MTZL：110.7 +/- 2.4 mmHg vs VEH：44.3 +/- 5.9 mmHg；P
OBJECTIVES: Type 2 diabetes causes mitochondrial dysfunction, which increases myocardial susceptibility to ischaemia-reperfusion injury. We investigated the efficacy of transplantation of mitochondria isolated from diabetic or non-diabetic donors in providing cardioprotection from warm global ischaemia and reperfusion in the diabetic rat heart.METHODS: Ex vivo perfused hearts from Zucker diabetic fatty (ZDF fa/fa) rats (n=6 per group) were subjected to 30min of warm global ischaemia and 120min reperfusion. Immediately prior to reperfusion, vehicle alone (VEH) or vehicle containing mitochondria isolated from either ZDF (MTZDF) or non-diabetic Zucker lean (ZL +/?) (MTZL) skeletal muscle were delivered to the coronary arteries via the aortic cannula.RESULTS: Following 30-min global ischaemia and 120-min reperfusion, left ventricular developed pressure was significantly increased in MTZDF and MTZL groups compared to VEH group (MTZDF: 92.85.2 mmHg vs MTZL: 110.7 +/- 2.4 mmHg vs VEH: 44.3 +/- 5.9 mmHg; P