ENHANCED BIOLOGICAL ACTIVITIES OF LAMINARIN DEGRADED BY GAMMA-RAY IRRADIATION
ENHANCED BIOLOGICAL ACTIVITIES OF LAMINARIN DEGRADED BY GAMMA-RAY IRRADIATION
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DOI：
10.1111/j.1745-4514.2011.00552.x
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发表时间：
2012-08-01
影响因子：
4
通讯作者：
Lee, Ju-Woon
中科院分区：
文献类型：
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作者：
Choi, Jong-Il;Kim, Hyun-Joo;Lee, Ju-Woon
在这项研究中，我们研究了γ辐照层粘胶蛋白的抗氧化和黑色素合成抑制特性。通过铁还原抗氧化电位值和1,1-二苯基-2-苦味酰肼自由基清除活性测定，发现辐照层状蛋白的抗氧化活性高于未辐照层状蛋白。然而，未辐照和辐照层状蛋白在β -胡萝卜素漂白试验中没有显着差异。此外，伽玛辐照的层粘连素增加了B16BL6黑色素瘤细胞对黑色素合成的抑制活性。随着辐照剂量的增加，层压素中还原糖的含量增加。这些结果和凝胶渗透色谱法测定的分子量表明，层粘连蛋白是通过糖苷键的断裂而被降解为低分子量的形式。总之，低分子量层粘胶蛋白可能具有有益的生物学特性，伽马辐射可能是产生低分子量层粘胶蛋白的有效方法。海带多糖是从海藻中提取的一种贮藏多糖。由于其在上消化道不易消化，层叠蛋白被认为是一种膳食纤维。层粘连蛋白还具有其他有益的生物学特性，但研究表明这些特性是无效的。因此，在本研究中，通过伽马辐射降解层板蛋白以增强这些生物学特性的影响。我们观察到伽玛辐照的层叠蛋白比未辐照的层叠蛋白具有更高的抗氧化活性和黑色素合成抑制活性。这项研究的结果表明，层粘胶蛋白可能提供饮食益处，而不仅仅是作为一种有效的膳食纤维。
In this study, we investigated the antioxidant and melanin synthesis inhibitory properties of gamma-irradiated laminarin. Antioxidant activities as measured by ferric-reducing antioxidant potential values and 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity were found to be greater in irradiated laminarin than in nonirradiated laminarin. However, nonirradiated and irradiated laminarins did not exhibit significant differences in beta-carotene bleaching assays. Moreover, inhibitory activity against melanin synthesis in B16BL6 melanoma cells was increased by gamma-irradiated laminarin. The content of reducing sugars in laminarin was found to increase with increased irradiation doses. These results and the molecular weight measured by gel permeation chromatography indicate that laminarin is degraded to a low molecular weight form by the scission of glycosidic bonds. Overall, low molecular weight laminarin may have beneficial biological properties, and gamma irradiation may be an effective method of producing low molecular weight laminarin. PRACTICAL APPLICATIONS Laminarin is a type of storage polysaccharide derived from seaweed. Because of its indigestibility in the upper gastrointestinal tract, laminarin is considered as a dietary fiber. Other beneficial biological properties have been attributed to laminarin, but studies have shown these properties to be ineffective. Therefore, in this study, laminarin was degraded by gamma irradiation to enhance the effects of these biological properties. We observed that gamma-irradiated laminarin had higher antioxidative and melanin synthesis inhibitory activities than nonirradiated laminarin did. The results of this study suggest that laminarin may provide dietary benefits beyond its use as an effective dietary fiber.