A disposable acoustofluidic chip for nano/microparticle separation using unidirectional acoustic transducers
A disposable acoustofluidic chip for nano/microparticle separation using unidirectional acoustic transducers
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DOI：
10.1039/d0lc00106f
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发表时间：
2020-04-07
期刊：
影响因子：
6.1
通讯作者：
Huang, Tony Jun
中科院分区：
文献类型：
--
作者：
Zhao, Shuaiguo;Wu, Mengxi;Huang, Tony Jun
基于表面声波（SAW）的纳米/微米颗粒分离在从样品纯化到癌症诊断的生物、化学和医学应用中显示出巨大的前景。然而，将微通道永久结合到相对昂贵的压电基板和激励换能器上使得SAW分离装置不可丢弃。这种限制不仅需要繁琐的清洁和增加的劳动力和材料成本，而且还导致交叉污染，阻止它们在许多生物，化学和医学应用中的实施。在这里，我们展示了一个高性能的，一次性的纳米/微粒分离的声流体平台。利用单向叉指换能器（IDT）、具有硬/软材料的混合通道设计以及倾斜角站立SAW（taSSAW），我们的一次性声流器件实现了与现有永久粘合的非一次性器件产生的声辐射力相当的声辐射力。我们的一次性设备不仅可以分离微粒，还可以分离纳米颗粒。此外，它们可以将细菌与纯度高达96%的人类红细胞（RBC）区分开来。总之，我们开发了一种单向IDT为基础的，一次性的微/纳米粒子分离的声流控平台，可以实现高分离效率，多功能性和生物相容性。
Separation of nano/microparticles based on surface acoustic waves (SAWs) has shown great promise for biological, chemical, and medical applications ranging from sample purification to cancer diagnosis. However, the permanent bonding of a microchannel onto relatively expensive piezoelectric substrates and excitation transducers renders the SAW separation devices non-disposable. This limitation not only requires cumbersome cleaning and increased labor and material costs, but also leads to cross-contamination, preventing their implementation in many biological, chemical, and medical applications. Here, we demonstrate a high-performance, disposable acoustofluidic platform for nano/microparticle separation. Leveraging unidirectional interdigital transducers (IDTs), a hybrid channel design with hard/soft materials, and tilted-angle standing SAWs (taSSAWs), our disposable acoustofluidic devices achieve acoustic radiation forces comparable to those generated by existing permanently bonded, non-disposable devices. Our disposable devices can separate not only microparticles but also nanoparticles. Moreover, they can differentiate bacteria from human red blood cells (RBCs) with a purity of up to 96%. Altogether, we developed a unidirectional IDT-based, disposable acoustofluidic platform for micro/nanoparticle separation that can achieve high separation efficiency, versatility, and biocompatibility.