Novel rapidly evolving hominid RNAs bind nuclear factor 90 and display tissue-restricted distribution.
Novel rapidly evolving hominid RNAs bind nuclear factor 90 and display tissue-restricted distribution.
复制标题
迅速发展的人类RNA结合核因子90并显示组织限制的分布。
DOI：
10.1093/nar/gkm668
复制
发表时间：
2007
影响因子：
14.9
通讯作者：
Mathews, Michael B
中科院分区：
文献类型：
--
作者：
Parrott, Andrew M;Mathews, Michael B
核因子90（NF 90）是一种双链RNA结合蛋白，参与多种细胞功能，但很少有确定的RNA伴侣。使用体内交联，然后免疫沉淀，我们发现了一个家庭的小NF 90相关的RNA（snaR）。这些高度结构化的117个核苷酸的非编码RNA在不同谱系的永生化人类细胞系中表达。在人体组织中，它们在睾丸中含量丰富，在脑、胎盘和其他一些器官中也有少量分布。从人293细胞中分离出两个snaR亚群，并通过生物信息学分析发现了其他物种。它们的基因通常以多个拷贝出现，排列在19号染色体上串联重复序列的两个反向区域。snaR-A由RNA聚合酶III从基因内启动子转录，快速翻转，并与Alu RNA和两种潜在的piRNA共享序列同一性。它与NF 90的双链RNA结合基序相互作用。snaR直向同源物存在于黑猩猩中，但不存在于其它哺乳动物中，并且包括位于两个绒毛膜促性腺激素基因的启动子中的基因。类人猿的snaRs似乎经历了加速进化和差异扩张。
Nuclear factor 90 (NF90) is a double-stranded RNA-binding protein implicated in multiple cellular functions, but with few identified RNA partners. Using in vivo cross-linking followed by immunoprecipitation, we discovered a family of small NF90-associated RNAs (snaR). These highly structured non-coding RNAs of ∼117 nucleotides are expressed in immortalized human cell lines of diverse lineages. In human tissues, they are abundant in testis, with minor distribution in brain, placenta and some other organs. Two snaR subsets were isolated from human 293 cells, and additional species were found by bioinformatic analysis. Their genes often occur in multiple copies arranged in two inverted regions of tandem repeats on chromosome 19. snaR-A is transcribed by RNA polymerase III from an intragenic promoter, turns over rapidly, and shares sequence identity with Alu RNA and two potential piRNAs. It interacts with NF90's double-stranded RNA-binding motifs. snaR orthologs are present in chimpanzee but not other mammals, and include genes located in the promoter of two chorionic gonadotropin hormone genes. snaRs appear to have undergone accelerated evolution and differential expansion in the great apes.