Genome-wide QTL mapping of saltwater tolerance in sibling species of Anopheles (malaria vector) mosquitoes.
Genome-wide QTL mapping of saltwater tolerance in sibling species of Anopheles (malaria vector) mosquitoes.
复制标题
按蚊（疟疾媒介）同胞物种盐水耐受性的全基因组 QTL 定位。
DOI：
10.1038/hdy.2015.39
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发表时间：
2015
期刊：
影响因子：
3.8
通讯作者：
Besansky,NJ
中科院分区：
文献类型：
--
作者：
Smith,HA;White,BJ;Kundert,P;Cheng,C;Romero-Severson,J;Andolfatto,P;Besansky,NJ
尽管淡水（FW）是蚊子幼虫的祖先栖息地，但多个物种独立进化出了在咸水（SW）中生存的能力。在这里，我们使用数量性状基因座（QTL）作图来研究按蚊（人类疟疾的传播媒介）渗透调节的遗传结构。我们分析了专性 FW 物种 An 之间杂交的 1134 个回交后代。 coluzzii 及其密切相关的广盐性同胞物种 An。梅鲁斯。通过贝叶斯区间作图和机器学习（随机森林）对 2387 个标记进行测试，得出了与 SW 耐受性相关的 6 个基因组区域。两种方法的 QTL 区域重叠增强了 QTL 鉴定的可信度。有证据表明基因座之间存在协同和破坏性上位性。有趣的是，一个含有离子转运蛋白的 QTL 区域跨越了区分这些物种的 2Rop 染色体倒位。我们的数据不是由一个或几个基因座控制的简单性状，而是与复杂的多基因遗传模式最一致。
Although freshwater (FW) is the ancestral habitat for larval mosquitoes, multiple species independently evolved the ability to survive in saltwater (SW). Here, we use quantitative trait locus (QTL) mapping to investigate the genetic architecture of osmoregulation in Anopheles mosquitoes, vectors of human malaria. We analyzed 1134 backcross progeny from a cross between the obligate FW species An. coluzzii, and its closely related euryhaline sibling species An. merus. Tests of 2387 markers with Bayesian interval mapping and machine learning (random forests) yielded six genomic regions associated with SW tolerance. Overlap in QTL regions from both approaches enhances confidence in QTL identification. Evidence exists for synergistic as well as disruptive epistasis among loci. Intriguingly, one QTL region containing ion transporters spans the 2Rop chromosomal inversion that distinguishes these species. Rather than a simple trait controlled by one or a few loci, our data are most consistent with a complex, polygenic mode of inheritance.