Effect of light intensity and nitrogen starvation on CO2 fixation and lipid/carbohydrate production of an indigenous microalga Scenedesmus obliquus CNW-N
Effect of light intensity and nitrogen starvation on CO2 fixation and lipid/carbohydrate production of an indigenous microalga Scenedesmus obliquus CNW-N
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DOI：
10.1016/j.biortech.2011.11.133
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发表时间：
2012-06-01
影响因子：
11.4
通讯作者：
Chang, Jo-Shu
中科院分区：
文献类型：
--
作者：
Ho, Shih-Hsin;Chen, Chun-Yen;Chang, Jo-Shu
应用工程策略来提高栅藻CNW-N分离物的CO2固定率和碳水化合物/脂质产生。确定了促进细胞生长、碳水化合物/脂质生产率和CO2固定效率的光强度。氮饥饿也被用来触发脂肪和碳水化合物的积累。生物量、脂肪和碳水化合物的最高生产率分别为840.57 mg L-1d（-1）、140.35 mg L-1d（-1）。脂肪和碳水化合物含量最高，分别为22.4%（饥饿5天）和46.65%（饥饿1天）。最佳CO2消耗速率为1420.6mg·L-1·d-1。这一表现优于大多数其他研究报告。在氮饥饿条件下，微藻油脂主要由C16/C18脂肪酸组成（约90%），适合于生物柴油的合成。存在于生物质中的碳水化合物主要是葡萄糖，占总碳水化合物的77-80%。这种碳水化合物组合物也适用于发酵生物燃料生产（例如，生物乙醇和生物丁醇）。(C)2011爱思唯尔有限公司保留所有权利。
Engineering strategies were applied to improve the CO2 fixation rate and carbohydrate/lipid production of a Scenedesmus obliquus CNW-N isolate. The light intensity that promotes cell growth, carbohydrate/lipid productivity, and CO2 fixation efficiency was identified. Nitrogen starvation was also employed to trigger the accumulation of lipid and carbohydrate. The highest productivity of biomass, lipid, and carbohydrate was 840.57 mg L-1 d(-1), 140.35 mg L-1 d(-1). The highest lipid and carbohydrate content was 22.4% (5-day N-starvation) and 46.65% (1-day N-starvation), respectively. The optimal CO2 consumption rate was 1420.6 mg L-1 d(-1). This performance is better than that reported in most other studies. Under nitrogen starvation, the microalgal lipid was mainly composed of C16/C18 fatty acid (around 90%), which is suitable for biodiesel synthesis. The carbohydrate present in the biomass was mainly glucose, accounting for 77-80% of total carbohydrates. This carbohydrate composition is also suitable for fermentative biofuels production (e.g., bioethanol and biobutanol). (C) 2011 Elsevier Ltd. All rights reserved.