Ca2+ channels activated by endothelin-1 in CHO cells expressing endothelin-A or endothelin-B receptors
Ca2+ channels activated by endothelin-1 in CHO cells expressing endothelin-A or endothelin-B receptors
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DOI：
10.1152/ajpcell.2001.281.5.c1676
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发表时间：
2001-11-01
影响因子：
5.5
通讯作者：
Masaki, T
中科院分区：
文献类型：
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作者：
Kawanabe, Y;Okamoto, Y;Masaki, T
我们使用Ca2+通道阻滞剂LOE-908和SK&F-96365比较了内皮素-1 （ET-1）在稳定表达内皮素A （ETA）或内皮素B （ETB）受体的中国仓鼠卵巢（CHO）细胞中激活的Ca2+通道。在CHO-ETA和CHO-ETB中，ET-1在0.1 nM激活Ca2+可渗透非选择性阳离子通道-1 (NSCC-1)，该通道对LOE-908敏感，对SK&F-96365耐药。ET-1在1 nM激活NSCC-2和NSCC-1；NSCC-2对LOE-908和SK&F-96365均敏感。在两种细胞类型中，10 nM ET-1激活的通道与1 nM ET-1激活的通道相同，但在CHO-ETA中，它额外激活了储存操作的Ca2+通道（SOCC），该通道对LOE-908具有抗性，对SK&F-96365敏感。在1 nM ET-1时，两种细胞类型的肌醇磷酸（IPs）的形成水平较低且相似，但在10 nM ET-1时，CHO-ETA的形成水平远高于CHO-ETB。这些结果表明，在CHO-ETA和CHO-ETB中，ET-1激活的Ca2+进入通道相同，0.1 nM ET-1激活NSCC-1，大于或等于1 nM的ET-1激活NSCC-1和NSCC-2。值得注意的是，在CHO-ETA中，10 nM ET-1激活了SOCCs，因为IPs的形成更高。
We compared the Ca2+ channels activated by endothelin-1 (ET-1) in Chinese hamster ovary (CHO) cells stably expressing endothelin type A (ETA) or endothelin type B (ETB) receptors using the Ca2+ channel blockers LOE-908 and SK&F-96365. In both CHO-ETA and CHO-ETB, ET-1 at 0.1 nM activated the Ca2+ permeable nonselective cation channel-1 (NSCC-1), which was sensitive to LOE-908 and resistant to SK&F-96365. ET-1 at 1 nM activated NSCC-2 in addition to NSCC-1; NSCC-2 was sensitive to both LOE-908 and SK&F-96365. ET-1 at 10 nM activated the same channels as 1 nM ET-1 in both cell types, but in CHO-ETA, it additionally activated the store-operated Ca2+ channel (SOCC), which was resistant to LOE-908 and sensitive to SK&F-96365. Up to 1 nM ET-1, the level of the formation of inositol phosphates (IPs) was low and similar in both cell types, but, at 10 nM ET-1, it was far greater in CHO-ETA than in CHO-ETB. These results show that, in CHO-ETA and CHO-ETB, ET-1 up to 10 nM activated the same Ca2+ entry channels: 0.1 nM ET-1 activated NSCC-1, and ET-1 greater than or equal to 1 nM activated NSCC-1 and NSCC-2. Notably, in CHO-ETA, 10 nM ET-1 activated SOCCs because of the higher formation of IPs.