Gestational and hormonal regulation of human placental lipoprotein lipase
Gestational and hormonal regulation of human placental lipoprotein lipase
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DOI：
10.1194/jlr.m600098-jlr200
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发表时间：
2006-11-01
影响因子：
6.5
通讯作者：
Powell, T. L.
中科院分区：
文献类型：
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作者：
Magnusson-Olsson, A. L.;Hamark, B.;Powell, T. L.
随着妊娠的进展，胎儿对 FFA 的需求增加，而 LPL 代表了增加胎盘脂质转运的一种潜在机制。我们检测了妊娠早期和足月人胎盘中的 LPL 活性和蛋白质表达。与妊娠早期 (n = 6) 胎盘相比，足月 (n = 7；P < 0.05) 的 LPL 活性高 3 倍。与足月 (n = 2) 胎盘相比，妊娠早期 (n = 2) 的微绒毛膜中 LPL 表达较低。我们将分离的胎盘绒毛碎片与多种效应物 [GW 1929、雌二醇、胰岛素、皮质醇、肾上腺素、胰岛素样生长因子-1 (IGF-1) 和肿瘤坏死因子-α] 孵育 1、3 和 24 小时，以研究潜在的调节机制。与雌二醇 (1 μg/ml)、胰岛素、皮质醇和 IGF-1 孵育 24 小时后，观察到 LPL 活性降低（n = 12；P，0.05）。我们观察到与雌二醇（20 ng/ml）或高血糖培养基加胰岛素孵育 3 小时后 LPL 活性增加（n = 7；P，0.05）。总之，我们认为妊娠期胎盘 LPL 活性的增加是妊娠晚期增强胎盘 FFA 转运的重要机制。胰岛素、皮质醇、IGF-1 和雌二醇对胎盘 LPL 活性的激素调节可能与妊娠变化以及妊娠期 LPL 活性的改变有关，并伴有胎儿生长的改变。
The fetal demand for FFA increases as gestation proceeds, and LPL represents one potential mechanism for increasing placental lipid transport. We examined LPL activity and protein expression in first trimester and term human placenta. The LPL activity was 3-fold higher in term (n = 7; P < 0.05) compared with first trimester (n = 6) placentas. The LPL expression appeared lower in microvillous membrane from first trimester (n = 2) compared with term (n = 2) placentas. We incubated isolated placental villous fragments with a variety of effectors [GW 1929, estradiol, insulin, cortisol, epinephrine, insulin-like growth factor-1 (IGF-1), and tumor necrosis factor-alpha] for 1, 3, and 24 h to investigate potential regulatory mechanisms. Decreased LPL activity was observed after 24 h of incubation with estradiol (1 mu g/ml), insulin, cortisol, and IGF-1 (n = 12; P, 0.05). We observed an increase in LPL activity after 3 h of incubation with estradiol (20 ng/ml) or hyperglycemic medium plus insulin (n = 7; P, 0.05). To conclude, we suggest that the gestational increase in placental LPL activity represents an important mechanism to enhance placental FFA transport in late pregnancy. Hormonal regulation of placental LPL activity by insulin, cortisol, IGF-1, and estradiol may be involved in gestational changes and in alterations in LPL activity in pregnancies complicated by altered fetal growth.