Leptin regulates energetic tradeoffs between body fat and humoural immunity
Leptin regulates energetic tradeoffs between body fat and humoural immunity
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DOI：
10.1098/rspb.2005.3126
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发表时间：
2005-09-07
影响因子：
4.7
通讯作者：
Sakaria, S
中科院分区：
文献类型：
--
作者：
Demas, GE;Sakaria, S
建立免疫应答需要相对大量的能量投入，并且能量可用性的显著减少可以抑制免疫功能并可能增加疾病易感性。我们以前已经证明，通过部分手术脂肪切除术（LIPx）适度减少能量储存会损害西伯利亚仓鼠（Photopus sungorus）的体液免疫。在这里，我们测试的假设，LIPx诱导的免疫力下降介导的脂肪组织激素瘦素的变化。仓鼠接受双侧腹股沟白色脂肪组织的手术切除（IWATx）或假手术（Sham）。每组中有一半的动物接受含有鼠瘦素的渗透性微型泵（0.5 μ l h（-1），持续10天），而其余的动物接受仅含有载体的微型泵;所有动物随后用新抗原钥孔血蓝蛋白（KLH）进行攻击。一般而言，血清瘦素和抗KLH抗体彼此显著相关，较高水平通常表明免疫力增强。此外，与假手术动物相比，IWATx仓鼠的血清抗KLH IgG显著降低。然而，外源性瘦素减弱了LIPx诱导的免疫抑制，但不影响假手术动物的体液免疫。这些结果表明，能量供应的减少导致体液免疫功能受损，瘦素可以作为体脂和免疫之间的神经内分泌信号调节体液免疫反应。
Mounting an immune response requires a relatively substantial investment of energy and marked reductions in energy availability can suppress immune function and presumably increase disease susceptibility. We have previously demonstrated that a moderate reduction in energy stores via partial surgical lipectomy (LIPx) impairs humoural immunity of Siberian hamsters (Phodopus sungorus). Here we tested the hypothesis that LIPx-induced decreases in immunity are mediated by changes in the adipose tissue hormone leptin. Hamsters received bilateral surgical removal of inguinal white adipose tissue (IWATx) or sham surgeries (Sham). Half the animals in each group received osmotic minipumps containing murine leptin (0.5 mu l h(-1) for 10 days) whereas the remaining animals received minipumps containing vehicle alone; all animals were subsequently challenged with the novel antigen keyhole limpet haemocyanin (KLH). In general, serum leptin and anti-KLH antibodies were significantly correlated with one another with higher levels generally indicating enhanced immunity. In addition, IWATx hamsters had significantly lower serum anti-KLH IgG compared with sham animals. Exogenous leptin, however, attenuated LIPx-induced immune suppression but did not affect humoural immunity in sham animals. These results suggest that reductions in energy availability lead to impairments in humoural immunity and that leptin can serve as a neuroendocrine signal between body fat and immunity regulating humoural immune responses.