Tumor necrosis factor alpha mRNA and protein are present in human placental and uterine cells at early and late stages of gestation.
Tumor necrosis factor alpha mRNA and protein are present in human placental and uterine cells at early and late stages of gestation.
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发表时间：
1991-08
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通讯作者：
Hua-lin Chen;Ya-ping Yang;Xiao-Ling Hu;K. Yelavarthi;J. Fishback;J. Hunt
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作者：
Hua-lin Chen;Ya-ping Yang;Xiao-Ling Hu;K. Yelavarthi;J. Fishback;J. Hunt
肿瘤坏死因子α（TNF-α）是一种调节细胞生长和调节各种细胞表面和分泌分子合成的多肽，已在妊娠子宫中鉴定。为了确定哪些特定细胞转录和翻译该基因，使用生物素标记的反义和正义TNF-α探针通过原位杂交分析了妊娠早期和晚期的胚胎外胎儿组织（胎盘和膜）和子宫组织的TNF-α mRNA，并使用两种单克隆抗体分析了免疫反应性TNF-α。在胚外和母体细胞中均鉴定出肿瘤坏死因子-α转录物和蛋白质。在孕早期胎盘绒毛中，TNF-α mRNA存在于合体滋养层，但在细胞滋养层和绒毛间质细胞中低至不存在。母体组织中的蜕膜和上皮细胞含有TNF-α转录本。在足月胎盘中，合体滋养层细胞和绒毛间质细胞均含有TNF-α mRNA，并且转录本存在于胎盘外膜附近的蜕膜中的母体细胞中。在孕早期和足月组织中，TNF-α mRNA和免疫反应性TNF-α的表达一致。这项研究的结果表明，人类妊娠期间胚外膜和母体组织中的细胞都会合成TNF-α，并且特定类型细胞的转录受到胎龄的影响。这些观察结果与TNF-α在人类妊娠动态发育事件中的主要作用一致。
Tumor necrosis factor alpha (TNF-alpha), a polypeptide that regulates cellular growth and modulates the synthesis of various cell surface and secreted molecules, has been identified in the pregnant uterus. To determine which specific cells transcribed and translated this gene, extraembryonic fetal tissues (placenta and membranes) and uterine tissue from early and late stages of gestation were analyzed for TNF-alpha mRNA by in situ hybridization using biotinylated antisense and sense TNF-alpha probes, and for immunoreactive TNF-alpha using two monoclonal antibodies. Tumor necrosis factor-alpha transcripts and protein were identified in both extraembryonic and maternal cells. In first-trimester placental villi, TNF-alpha mRNA was present in syncytiotrophoblast but was low to absent in cytotrophoblast and villous stromal cells. Decidual and epithelial cells in maternal tissues contained TNF-alpha transcripts. In term placentas, both syncytiotrophoblast and villous stromal cells contained TNF-alpha mRNA, and transcripts were present in maternal cells in the decidua adjacent to the extraplacental membranes. In both first-trimester and term tissues, coincident expression of TNF-alpha mRNA and immunoreactive TNF-alpha was demonstrated. The results of this study show that TNF-alpha is synthesized by cells in both extraembryonic membranes and maternal tissues during human gestation and that transcription in specific types of cells is influenced by gestational age. These observations are consistent with a major role for TNF-alpha in the dynamic developmental events of human pregnancy.