Fluoroquinolone resistance and DNA gyrase mutations in Salmonella Isolated from Chickens
Fluoroquinolone resistance and DNA gyrase mutations in Salmonella Isolated from Chickens
复制标题
DOI：
--
复制
发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Liu Fang-ping;Q. Peng
中科院分区：
文献类型：
--
作者：
Liu Fang-ping;Q. Peng
PCR扩增9株临床分离沙门菌对5种氟喹诺酮类药物（环丙沙星、氧氟沙星、恩诺沙星、达诺沙星、沙拉沙星）耐药的沙门氏菌gyrA和gyrB基因的QRDR区。与NCTC5776参考株序列比对发现，菌株38的gyrA 371 bp处出现C位点突变为T位点，A位点突变为C位点。菌株60的gyrA中存在350 bp的突变，而其他菌株的gyrA中未发现突变。该突变导致菌株38中Arg99Cys的氨基酸替换和菌株60中Met92Leu的氨基酸替换。菌株42的gyrB中1 592 bp处发现C到A的单突变，该突变位于gyrB QRDR之外，没有导致氨基酸变化。这些结果表明，DNA旋转酶的gyrA和gyrB的QRDR突变可能在沙门氏菌的氟喹诺酮耐药性中起不显着的作用。
The QRDR regions of gyrA and gyrB genes were amplified by PCR from 9 clinically isolated Salmonella strains showing resistant to 5 fluoroquinolones(Ciprofloxacin,Ofloxacin,Enrofloxacin,Danofloxacin and Sarafloxacin).Sequence comparison with NCTC5776 reference strain showed a site mutation from C to T at 371 bp in gyrA of strain 38 and a mutation from A to C at 350 bp in gyrA of strain 60.However no mutation was found in gyrA of other isolates.The mutation resulted in an amino acid substitution of Arg99Cys in strain 38 and an amino acid substitution of Met92Leu in strain 60.A single mutation from C to A was found at 1 592 bp in gyrB of strain 42,which was located outside of gyrB QRDR and led to no amino acid change. These results indicated that mutations in QRDR of gyrA and gyrB of DNA gyrase may play an insignificant role in fluoroquinolone resistance in Salmonella.