Sex Matters: Male Hamsters Are More Susceptible to Lethal Infection with Lower Doses of Pathogenic Leptospira than Female Hamsters
Sex Matters: Male Hamsters Are More Susceptible to Lethal Infection with Lower Doses of Pathogenic Leptospira than Female Hamsters
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DOI：
10.1128/iai.00369-18
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发表时间：
2018-10-01
影响因子：
3.1
通讯作者：
Gomes-Solecki, Maria
中科院分区：
文献类型：
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作者：
Gomes, Charles K.;Guedes, Mariana;Gomes-Solecki, Maria
已发表的证据表明，性别影响人类钩端螺旋体病的严重程度。在本研究中，我们采用急性疾病动物模型，分析了低剂量但不断增加的哥本哈根钩端螺旋体血清感染的雄性和雌性仓鼠的钩端螺旋体病。我们发现雄性仓鼠对钩端螺旋体病更敏感，在相同感染剂量下，只有6.3%的雄性仓鼠存活，而雌性仓鼠的存活率为68.7%。与雌性相比，雄性仓鼠在暴露于低感染剂量（类似于10(3)个细菌）后，肾脏中有较高的疑问乳杆菌负荷和较高的组织病理学评分。在感染高剂量疑问乳杆菌（类似于10(4)细菌）的仓鼠中，病原体负荷以及肾脏细胞因子和纤维化转录物水平的差异在性别之间没有明显差异。结果表明，感染了疑问乳杆菌的雄性仓鼠在暴露于较低的感染剂量后比雌性仓鼠更容易发生严重的钩端螺旋体病。
A somewhat contradictory published body of evidence suggests that sex impacts severity outcomes of human leptospirosis. In this study, we used an acute animal model of disease to analyze leptospirosis in male and female hamsters infected side by side with low but increasing doses of Leptospira interrogans serovar Copenhageni. We found that male hamsters were considerably more susceptible to leptospirosis, given that only 6.3% survived infection, whereas 68.7% of the females survived the same infection doses. In contrast to the females, male hamsters had high burdens of L. interrogans in kidney and high histopathological scores after exposure to low infection doses (similar to 10(3) bacteria). In hamsters infected with higher doses of L. interrogans (similar to 10(4) bacteria), differences in pathogen burdens as well as cytokine and fibrosis transcript levels in kidney were not distinct between sexes. Our results indicate that male hamsters infected with L. interrogans are more susceptible to severe leptospirosis after exposure to lower infectious doses than females.