Acupuncture at heterotopic acupoints facilitates distal colonic motility via activating M3 receptors and somatic afferent C-fibers in normal, constipated, or diarrhoeic rats
Acupuncture at heterotopic acupoints facilitates distal colonic motility via activating M3 receptors and somatic afferent C-fibers in normal, constipated, or diarrhoeic rats
复制标题
针刺异位穴位通过激活正常、便秘或腹泻大鼠的 M3 受体和体细胞传入 C 纤维促进远端结肠运动
DOI：
10.1111/nmo.12694
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发表时间：
2015-12-01
影响因子：
3.5
通讯作者：
Zhu, B.
中科院分区：
文献类型：
--
作者：
Gao, X.;Qin, Q.;Zhu, B.
背景既往研究证实了体细胞刺激对胃肠动力障碍患者的有效性。然而，很少有人研究针灸对结肠动力的影响，特别是在病理条件下。在调节针刺对结肠运动的确切机制仍然不清楚。MethodsWe评估的影响，针刺在异位穴位对远端结肠运动使用温水填充的测压气球插入5-6厘米到直肠的麻醉正常大鼠或大鼠腹泻或便秘。氯化胆碱，4-DAMP，眼镜蛇毒和辣椒素分别施加到调查的作用，M-3受体在调节远端结肠运动的针刺在异位穴位，以及是否A-和/或C-纤维需要触发远端结肠运动由acupuncture.Key ResultsThe远端结肠运动针刺在异位穴位不仅在正常大鼠，但也在便秘或腹泻的大鼠。M-3受体在针刺异位穴引起的远端结肠运动易化中起重要作用。传入神经A-和C-纤维介导的针刺信号的转导和C-纤维是必不可少的，以加强在异位穴位针刺对远端colonicmotority.Conclusions和InferencesOur结果显示，针刺异位穴位增加远端colonicmotority. Conclusions的效果，无论正常或病理条件下，通过主要激活C-纤维的躯体传入神经和M-3受体。
BackgroundPrevious studies have demonstrated the efficacy of somatic stimulation for patients with gastrointestinal motility disorders. However, little effort has been made to investigate the effects of acupuncture on colonic motility, particularly in pathological conditions. The precise mechanism employed in the regulation of acupuncture on colonic motility still remains unclear.MethodsWe assessed the effect of acupuncture at heterotopic acupoints on distal colonic motility using a warm-water-filled manometric balloon inserted 5-6 cm into the rectum of anesthetized normal rats or rats with diarrhea or constipation. Choline chloride, 4-DAMP, cobra venom and capsaicin were separately applied to investigate the role of M-3 receptors in the regulation of distal colonic motility by acupuncture at heterotopic acupoints, and whether A- and/or C-fibers are required for triggering distal colonic motility by acupuncture.Key ResultsAcupuncture at heterotopic acupoints increased distal colonic motility not only in normal rats but also in rats with constipation or diarrhea. M-3 receptors play an important role in the facilitation of distal colonic motility triggered by acupuncture at heterotopic acupoints. Afferent nerve A- and C-fibers mediate the transduction of the acupuncture signal and C-fibers are essential for enhancing the effect of acupuncture at the heterotopic acupoint on distal colonic motility.Conclusions & InferencesOur results reveal that acupuncture at heterotopic acupoints increases distal colonic motility regardless of normal or pathological conditions via predominately activating C-fibers of somatic afferent nerve and M-3 receptors.