Proteomic Analysis Reveals Growth Inhibition of Coriolus versicolor by Methanol Extracts of Cinnamomum camphora Xylem
Proteomic Analysis Reveals Growth Inhibition of Coriolus versicolor by Methanol Extracts of Cinnamomum camphora Xylem
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蛋白质组学分析揭示樟树木质部甲醇提取物对云芝生长的抑制作用
DOI：
10.1155/2021/6337906
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发表时间：
2021-08
影响因子：
3.3
通讯作者：
Lin Hui
中科院分区：
文献类型：
--
作者：
Li Quan;Li Xiangyang;Lin Hui
抗腐木树种提取物是今后木材防腐剂开发的重要研究对象。为了解樟树木质部甲醇提取物抑制杂色云芝的作用机理，采用双向凝胶电泳法、MALDI-TOF/MS和生物信息学方法对杂色云芝的蛋白质组分进行了分析。结果表明，香椿木质部甲醇提取物处理的366-385个蛋白点中，有41个蛋白点发生了明显的变化。21个蛋白质点上调，20个蛋白质点下调。对这些差异蛋白进行了细胞定位，生物学过程和功能分析发现其中9个在细胞质中，6个在细胞内，5个在线粒体中。小分子代谢过程占18.8%，细胞氨基酸代谢过程占12.5%，细胞含氮化合物代谢过程占10.9%。25%的差异蛋白与离子结合有关，15%与氧化还原酶活性相关，15%与ATPase活性和跨膜转运活性相关。天冬氨酸氨基转移酶、ATP合成酶α链、DEAD/Deah-box螺旋酶和磷酸甘油酸激酶的表达下调表明，樟树木质部的甲醇提取物扰乱了氮和碳代谢、能量代谢、激素信号反应和葡萄糖代谢等功能，最终导致杂色樟树的抑制。
The extracts of decay-resistant tree species are important research objects for the future development of wood preservatives. To understand the antifungal mechanisms of Coriolus versicolor inhibition with methanol extracts of C. camphora xylem, the protein profiles of C. versicolor were analyzed using 2-DE followed by MALDI-TOF/MS and bioinformatic analyses. The results showed that 41 protein spots were obviously changed among the 366-385 protein spots of C. versicolor treated with methanol extracts of C. camphora xylem. Twenty-one protein spots were upregulated, and 20 protein spots were downregulated. Cellular localization was performed to identify these differential proteins, and biological process and functional analysis found that 9 of these proteins were in the cytoplasm, 6 were intracellular, and 5 were in the mitochondrion. A total of 18.8% were mapped to small-molecule metabolic processes, 12.5% to cellular amino acid metabolic processes, and 10.9% to cellular nitrogen compound metabolic processes. Twenty-five percent of the differential proteins were associated with ion bonding, 15% with oxidoreductase activity, and 15% with ATPase activity and transmembrane transport activity. Downregulated expression of aspartate aminotransferase, ATP synthase alpha chain, DEAD/DEAH-box helicase, and phosphoglycerate kinase showed that the methanol extracts of C. camphora xylem disrupted functional aspects such as nitrogen and carbon metabolism, energy metabolism, hormone signal response, and glucose metabolism, eventually leading to C. versicolor inhibition.
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影响因子：
13.6
作者：
A. Janin;L. Coudert;P. Riché;G. Mercier;P. Cooper;J. Blais
通讯作者：
A. Janin;L. Coudert;P. Riché;G. Mercier;P. Cooper;J. Blais
影响因子：
2
作者：
Sun C;Song C;Ma Z;Xu K;Zhang Y;Jin H;Tong S;Ding W;Xia G;Ding Q
通讯作者：
Ding Q
影响因子：
2.9
作者：
D. Mandelman;L. Ballut;David A. Wolff;G. Feller;C. Gerday;R. Haser;N. Aghajari
通讯作者：
D. Mandelman;L. Ballut;David A. Wolff;G. Feller;C. Gerday;R. Haser;N. Aghajari
影响因子：
3
作者：
S. Kumari;P. Jain;B. Sharma;Preeti Kadyan;R. Dabur
通讯作者：
S. Kumari;P. Jain;B. Sharma;Preeti Kadyan;R. Dabur
影响因子：
0.7
作者：
Bhardwaj, Surender Kumar;Singla, Sushil Kumar;Bhardwaj, Rakesh Kumar
通讯作者：
Bhardwaj, Rakesh Kumar