An nhr-85::GFP::AID*::3xFLAG knock-in allele for investigation of molting and oscillatory gene regulation.
An nhr-85::GFP::AID*::3xFLAG knock-in allele for investigation of molting and oscillatory gene regulation.
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DOI：
10.17912/micropub.biology.000993
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发表时间：
2023
影响因子：
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通讯作者：
Ward, Jordan D
中科院分区：
文献类型：
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作者：
Myles, Krista M;Clancy, John C;Johnson, Londen C;Ashley, Guinevere;Manzano, Jesus;Ragle, James Matthew;Ward, Jordan D
线虫NHR-85是一种核激素受体转录因子，在调节microRNA表达以控制发育时机方面发挥着重要作用。我们通过将GFP：：AID*：：3xFLAG盒插入内源位置来标记所有已知的亚型，从而产生了第一个NHR-85翻译融合。NHR-85：：GFP：：AID*：：3xFLAG动物有野生型孵化，NHR-85：：GFP在上皮细胞L4阶段开始时表达高峰。NHR-85在胚系中不表达，这表明虽然它可能与NHR-23转录因子协同控制microRNA的表达，但NHR-23促进精子发生不依赖于NHR-85。这种NHR-85：：GFP：：AID*：：3xFLAG株将是研究NHR-85何时何地促进发育计时的宝贵资源。
C. elegans NHR-85 is a poorly characterized nuclear hormone receptor transcription factor with an emerging role in regulating microRNA expression to control developmental timing. We generated the first NHR-85 translational fusion by knocking a GFP::AID*::3xFLAG cassette into the endogenous locus to tag all known isoforms. nhr-85 ::GFP::AID*::3xFLAG animals have wild-type broodsizes and NHR-85 ::GFP peaks in expression at the start of the L4 stage in epithelial cells. NHR-85 is not expressed in the germline, suggesting that while it might cooperate with the NHR-23 transcription factor to control microRNA expression, NHR-23 promotes spermatogenesis independent of NHR-85 . This nhr-85 ::GFP::AID*::3xFLAG strain will be a valuable resource for studying when and where NHR-85 acts to promote developmental timing.