Exhaustive exercise modifies oxidative stress in smoking subjects
Exhaustive exercise modifies oxidative stress in smoking subjects
复制标题
DOI：
10.1097/maj.0b013e318065b57c
复制
发表时间：
2007-06-01
影响因子：
3.1
通讯作者：
Levy, Yishai
中科院分区：
文献类型：
--
作者：
Gochman, Einat;Reznick, Abraham Z.;Levy, Yishai
背景。力竭运动与代谢率增加和活性氧生成加速有关。香烟烟雾还含有可能参与动脉粥样硬化发展的氧化剂。然而，关于运动和吸烟之间关系的数据很少。方法：在标准最大运动测试前后，对30名健康、受过训练的年轻男性（15名吸烟者和15名非吸烟者，平均年龄23.7±2.6岁）进行了评估。结果：运动导致吸烟者（+17.7%,P < 0.001）和不吸烟者（+19.1%,P < 0.05）的蛋白质氧化（羰基测定）增加，运动后吸烟者与不吸烟者的血浆共轭二烯（+ 157%,P < 0.04）和血浆脂质过氧化物（+14%,P < 0.059）升高。与不吸烟者相比，吸烟组血浆抗氧化剂水平显著降低，总类胡萝卜素（-36.5%,P < 0.001）、维生素A （-80%, P < 0.001）和维生素E （-64%, P < 0.002）的含量减少。运动后，所有受试者的肌肉酶（CPK、LDH）和尿蛋白（微量白蛋白和肌红蛋白）的渗漏均显著增加。运动前后，吸烟者的CRP水平高于不吸烟者。结论：我们的研究结果表明，吸烟和剧烈运动之间存在未被注意到的相互作用，这表明吸烟者更容易受到氧化损伤，可能是由于抗氧化能力较低。
Background. Exhaustive exercise is associated with increased metabolic rate and accelerated generation of reactive oxygen species. Cigarette smoke also contains oxidants that may participate in the development of atherosclerosis. However, data on the association between exercise and smoking are sparse. Methods: A homogenous group of 30 young men (15 smokers and 15 nonsmokers; mean age, 23.7 +/- 2.6 years), healthy, trained subjects, were assessed before and after a standard maximal exercise test. Results: Exercise led to increased protein oxidation (carbonyl assay) in both smokers (+17.7%, P < 0.001) and nonsmokers (+19.1%, P < 0.05), elevation in plasma conjugated dienes (+ 157%, P < 0.04), and plasma lipid peroxides (+14%, P < 0.059) in smokersversus nonsmokers after exercise. Plasma antioxidants levels were significantly lower in the smoking group, with reduction in total carotenoids (-36.5%, P < 0.001), vitamin A (-80%, P < 0.001), and vitamin E (-64%, P < 0.002), compared with nonsmokers. A significant rise in leakage of muscle enzymes (CPK, LDH) and urine proteins (microalbumin and myoglobin) occurred in all subjects after exercise. CRP levels were higher in smokers compared with nonsmokers before and after exercise. Conclusions: Our results suggest that unnoticed interaction exists between smoking and intense exercise, which indicates that smokers are more susceptible to oxidative insults probably due to lower antioxidant capacity.