Effects of liquid nitrogen treatment on the proliferation of osteosarcoma and the biomechanical properties of normal bone.
Effects of liquid nitrogen treatment on the proliferation of osteosarcoma and the biomechanical properties of normal bone.
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DOI：
10.1007/s10776-002-0626-3
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发表时间：
2003-01-01
期刊：
影响因子：
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通讯作者：
Tomita, Katsuro
中科院分区：
文献类型：
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作者：
Yamamoto, Norio;Tsuchiya, Hiroyuki;Tomita, Katsuro
为了克服在存在恶性骨和软组织肿瘤的情况下进行自体移植重建的问题，我们设计了一种利用液氮的低温效应来治疗自体移植的方法。我们测量了每种情况下骨骼内温度的变化，并建立了一个周期的液氮方案，包括在液氮中20分钟，在室内空气中15分钟，在生理盐水中15分钟。用溴脱氧尿嘧啶核苷(BrdU)免疫组织化学染色检测液氮法处理的肿瘤细胞的增殖能力。在裸鼠体内检测肿瘤的增殖能力。根据结果，我们得出结论，肿瘤细胞的死亡是液氮方法的结果。在压缩强度方面，完整骨和液氮处理骨之间没有显著差异，而高压灭菌骨的强度有所降低。压缩试验后高压灭菌骨的断口扫描电子显微镜显示不规则、凹凸不平的表面，而经液氮处理的骨的断口表面光滑细小。这可能是抗压强度差异的原因之一。
To overcome problems of autografts for reconstruction in the presence of malignant bone and soft tissue tumors, we devised a method for treating autografts that utilizes the hypothermic effect of liquid nitrogen. We measured temperature changes inside the bone at each condition and established a one-cycle liquid nitrogen protocol that included 20 min in liquid nitrogen, 15 min in room air, and 15 min in physiological saline. The proliferation potential of the tumor cells treated with the liquid nitrogen method was examined by means of bromodeoxyuridine (BrdU) immunostaining. Tumor proliferation potential in vivo was examined in nude mice. Based on the results we concluded that the tumor cells died out as a result of the liquid nitrogen method. Regarding compression strength there was no significant difference between intact bone and liquid nitrogen-treated bone, whereas the strength of the autoclaved bone was decreased. Scanning electron microscopic examination of the fracture surface of the autoclaved bone after the compression test showed an irregular, uneven surface, whereas that of the liquid nitrogen-treated bone was smooth and fine-grained. This might be one of the reasons for the discrepancy in compression strength.