SURVIVAL OF VIBRIO-CHOLERAE AND ESCHERICHIA-COLI IN ESTUARINE WATERS AND SEDIMENTS
SURVIVAL OF VIBRIO-CHOLERAE AND ESCHERICHIA-COLI IN ESTUARINE WATERS AND SEDIMENTS
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DOI：
10.1128/aem.43.3.578-584.1982
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发表时间：
1982-01-01
影响因子：
4.4
通讯作者：
NESS, GE
中科院分区：
文献类型：
--
作者：
HOOD, MA;NESS, GE
用平板计数法和直接计数法测定了霍乱弧菌和大肠杆菌在河口水和沉淀池中的存活率。霍乱弧菌包括环境、临床和血清型O1型和非O1型分离株，而大肠杆菌株包括atcc 25922和一种新鲜培养的人分离株。霍乱弧菌的回收率随孵育温度的不同而有显著差异。在无菌沉积物、无菌水和非无菌水中生长和延长存活期，但在非无菌沉积物中没有。与霍乱弧菌不同的是，在无菌和无菌河口水域中，活菌迅速减少。直接计数显示，大肠杆菌细胞在河口水中完好无损，但试图复苏它们的努力失败了。这些数据表明，霍乱弧菌在河口水域中的生存能力要好于大肠杆菌。这些结果可能解释了最近的观察结果，即霍乱弧菌水平与河口水域的粪便大肠菌群浓度没有很好的相关性。此外，这些结果还增加了越来越多的证据，支持霍乱弧菌是河口本地细菌的理论。
In in vitro estuarine water and sediment chambers, the survival of Vibrio cholerae and Escherichia coli was determined by plate counting and direct counting techniques. V. cholerae strains included environmental, clinical, and serotype O1 and non-O1 isolates, whereas E. coli strains included ATCC 25922 and a freshly cultured human isolate. Recovery of V. cholerae varied significantly with incubation temperature. Growth and extended periods of survival occurred in sterile sediments, sterile waters, and nonsterile waters, but not in nonsterile sediments. In contrast to V. cholerae, viable cells of E. coli decreased rapidly in both sterile and nonsterile estuarine waters. Direct counts revealed that E. coli cells were intact in the estuarine water, but attempts to resuscitate them were unsuccessful. The data suggest that V. cholerae survives better in estuarine waters than E. coli. The results may explain the recent observations that V. cholerae levels do not correlate well with fecal coliform concentrations in estuarine waters. Furthermore, the results add increasing evidence to support the theory that V. cholerae is an autochthonous bacterium in estuaries.