Prostate stem cell antigen enhancer and uroplakin II promoter based bladder cancer targeted tissue-specific vector
Prostate stem cell antigen enhancer and uroplakin II promoter based bladder cancer targeted tissue-specific vector
复制标题
基于前列腺干细胞抗原增强剂和尿斑蛋白 II 启动子的膀胱癌靶向组织特异性载体
DOI：
10.1016/j.urolonc.2008.02.002
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发表时间：
2010-03-01
影响因子：
2.7
通讯作者：
Rodriguez, Ronald
中科院分区：
文献类型：
--
作者：
Wang, Degui;Wang, Zhiping;Rodriguez, Ronald
Purpose: To construct a dual specific vector which contains prostate stem cell antigen enhancer (PSCAE) and uroplakin II (UPII) promoter targeted bladder cancer.Methods: UPII promoter and PSCAE were amplified by polymerase chain reaction (PCR). Luciferase gene (LUC) was obtained from plasmid pBK-CMV-LUC. PSCAE. UPII promoter and LUC were inserted into shuttle plasmid to create Rp-UPII-LUC and Rp-PSCAE-UPII-LUC. Rp-UPII-LUC and Rp-PSCAE-UPII-LUC were cotransfected with pCMV-beta-gal into various cell lines at the presence or absence of androgen receptor agonist R1881 and androgen receptor antagonist flutamide. Luminescence was detected with luciferase assay kit and counted on liquid scintillation counter.Results: Bladder cancer cells showed higher LUC activity than non-bladder cancer cells after transfected with plasmids Rp-UPII-LUC and Rp-PSCAE-UPII-LUC. PSCAE could improve the LUC activity in both AR positive and AR negative bladder cancer cells but not in non-bladder cancer cells and normal human urothelial (NHU) cells. R1881 could increase the LUC activity in AR positive bladder cancer cells but not in AR negative bladder cancer cells and non-bladder cancer cells. Flutamide could not inactivate PSCAE in bladder cancer cells.Conclusions: PSCAE can improve target gene expression in bladder cancer cells but not in non-bladder cancer cells and NHU cells. PSCAE maintains a certain level of androgen independent activity in bladder cancer cells. PSCAE is active in both AR positive and AR negative bladder cancer cells. The results suggest that combination of PSCAE with UPII promoter is feasible in constructing bladder cancer-specific vectors. (C) 2010 Elsevier Inc. All rights reserved.
