Presence of activin (erythroid differentiation factor) in unfertilized eggs and blastulae of Xenopus laevis.
Presence of activin (erythroid differentiation factor) in unfertilized eggs and blastulae of Xenopus laevis.
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非洲爪蟾未受精卵和囊胚中存在激活素（红细胞分化因子）。
DOI：
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发表时间：
1991
影响因子：
11.1
通讯作者：
K. Kinoshita
中科院分区：
文献类型：
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作者：
M. Asashima;H. Nakano;H. Uchiyama;H. Sugino;Takanori Nakamura;Y. Eto;D. Ejima;S. Nishimatsu;N. Ueno;K. Kinoshita
激活素A是转化生长因子β超家族的成员之一，最近被发现对分离的非洲爪哇早期动物帽子细胞具有很强的中胚层诱导活性。我们通过与Friend白血病细胞的红系分化试验来测量非洲爪哇卵提取物的激活素活性。结果表明，非洲爪哇的未受精卵和囊胚中确实含有相当数量的激活素同系物。当用反相高效液相色谱分离时，该活性在与人激活素A相同的保留时间内洗脱。此外，含有红系分化因子活性的部分对非洲爪哇动物帽细胞具有中胚层诱导活性。当与激活素结合蛋白卵泡抑素共同孵育时，该组分的中胚层诱导活性被抑制。这些结果表明，一种内源性激活素可能是一种天然的中胚层诱导因子，在非洲爪哇胚胎发生中起作用。
Activin A, a member of the transforming growth factor beta superfamily, has recently been found to have potent mesoderm-inducing activity on isolated early Xenopus animal-cap cells. We measured the activin activity of the Xenopus egg extract by using an erythroid-differentiating test with Friend leukemia cells. The results showed that an activin homologue is, indeed, contained in unfertilized eggs and blastulae of Xenopus laevis in a considerable amount. This activity was eluted at the same retention time as human activin A when fractionated by reversed-phase HPLC. Furthermore, the fraction containing erythroid-differentiating factor activity had mesoderm-inducing activity on Xenopus animal-cap cells. The mesoderm-inducing activity of this fraction was suppressed when coincubated with follistatin, an activin-binding protein. These results suggest that an endogenous activin may be a natural mesoderm-inducing factor acting in Xenopus embryogenesis.