POLYMERASE CHAIN-REACTION WITH SINGLE-SIDED SPECIFICITY - ANALYSIS OF T-CELL RECEPTOR DELTA-CHAIN
POLYMERASE CHAIN-REACTION WITH SINGLE-SIDED SPECIFICITY - ANALYSIS OF T-CELL RECEPTOR DELTA-CHAIN
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DOI：
10.1126/science.2463672
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发表时间：
1989-01-13
期刊：
影响因子：
56.9
通讯作者：
DAVIS, MM
中科院分区：
文献类型：
--
作者：
LOH, EY;ELLIOTT, JF;DAVIS, MM
在聚合酶链反应（PCR）中，使用两个特异性寡核苷酸引物来扩增它们之间的序列。然而，该技术不适合扩增编码其中目的序列的5“部分未知的分子的基因，例如T细胞受体（TCR）或免疫球蛋白。由于这种限制，设计了一种新的技术，锚定聚合酶链反应（A-PCR），它只需要靶片段3“末端的序列特异性。将其用于分析TCR δ。来自人外周血的γ链mRNA。δ的T细胞。这些细胞中的大多数具有V δ。先前未描述的基因片段（V δ 3），以及δ链连接序列形成一个离散的亚群相比，以前报道的。
In the polymerase chain reaction (PCR), two specific oligonucleotide primers are used to amplify the sequences between them. However, this technique is not suitable for amplifying genes that encode molecules where the 5'' portion of the sequences of interest is not known, such as the T cell receptor (TCR) or immunoglobulins. Because of this limitation, a novel technique, anchored polymerase chain reaction (A-PCR), was devised that requires sequence specificity only on the 3'' end of the target fragment. It was used to analyze TCR .delta. chain mRNA''s from human peripheral blood .gamma..delta. T cells. Most of these cells had a V.delta. gene segment not previously described (V.delta.3), and the .delta. chain junctional sequences formed a discrete subpopulation compared with those previously reported.