Polarised light sheet tomography.
Polarised light sheet tomography.
复制标题
偏振光片断层扫描。
DOI：
10.1364/oe.24.011239
复制
发表时间：
2016
期刊：
影响因子：
3.8
通讯作者：
M. MacDonald
中科院分区：
文献类型：
--
作者：
Sascha Reidt;Daniel J. O'Brien;K. Wood;M. MacDonald
薄片显微镜的各种优点使其成为捕获三维图像的一种广泛使用的方式。它主要用于荧光成像，但最近出现了另一种仅依靠散射的技术，称为光片层析成像。该方法已成功地应用于透明土壤中植物根系的成像，但当涉及到更浑浊的样品时，它是有限的。本文介绍了偏振光片层析成像系统及其在高散射浑浊介质中成像的优势。实验配置由蒙特卡罗辐射传输方法指导，该方法模拟了样品中偏振光片的传播。在复杂的胶原基质中获得了反射和吸收幻影的图像，并比较了不同偏振配置的结果。然后使用焦点扫描方法来降低噪声并产生吸收目标的三维重建。
The various benefits of light sheet microscopy have made it a widely used modality for capturing three-dimensional images. It is mostly used for fluorescence imaging, but recently another technique called light sheet tomography solely relying on scattering was presented. The method was successfully applied to imaging of plant roots in transparent soil, but is limited when it comes to more turbid samples. This study presents a polarised light sheet tomography system and its advantages when imaging in highly scattering turbid media. The experimental configuration is guided by Monte Carlo radiation transfer methods, which model the propagation of a polarised light sheet in the sample. Images of both reflecting and absorbing phantoms in a complex collagenous matrix were acquired, and the results for different polarisation configurations are compared. Focus scanning methods were then used to reduce noise and produce three-dimensional reconstructions of absorbing targets.
影响因子：
3.4
作者：
Glaser, A. K.;Wang, Y.;Liu, J. T. C.
通讯作者：
Liu, J. T. C.
影响因子：
3.5
作者：
Jacques, SL;Ramella-Roman, JC;Lee, K
通讯作者：
Lee, K