Isolation and characterization of indigo-reducing bacteria and analysis of microbiota from indigo fermentation suspensions
Isolation and characterization of indigo-reducing bacteria and analysis of microbiota from indigo fermentation suspensions
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DOI：
10.1093/bbb/zbab209
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发表时间：
2022-01-24
影响因子：
1.6
通讯作者：
Sakuradani, Eiji
中科院分区：
文献类型：
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作者：
Nakagawa, Kasumi;Takeuchi, Michiki;Sakuradani, Eiji
在天然靛蓝染色中，堆肥的青叶中的水不溶的青蓝称为Sukumo，通过微生物在碱性条件下的还原活动转化为水溶性的无色青蓝。为了了解还原青蓝与发酵液中微生物的关系，我们分离和鉴定了还原青蓝的微生物，并分析了发酵液中的微生物区系。以靛蓝为直接底物，分离出了不是用常规的靛红还原试验方法分离到的还原青蓝微生物，并对其进行了进一步的鉴定和表征。我们成功地分离到与谷氨酸棒杆菌、金黄色葡萄球菌和肠球菌密切相关的细菌。这是第一次。研究发现，蒽醌是一种有效的介体物质，能促进分离菌株的还原活性。通过对青黛发酵悬浮液中微生物区系的分析，发现蓝绿还原菌与其他细菌的比例对维持青蓝还原活性具有重要意义。
In natural indigo dyeing, the water-insoluble indigo included in the composted indigo leaves called sukumo is converted to water-soluble leuco-indigo through the reduction activities of microorganisms under alkaline conditions. To understand the relationship between indigo reduction and microorganisms in indigo-fermentation suspensions, we isolated and identified the microorganisms that reduce indigo and analyzed the microbiota in indigo-fermentation suspensions. Indigo-reducing microorganisms, which were not isolated by means of a conventional indigo carmine-reduction assay method, were isolated by using indigo as a direct substrate and further identified and characterized. We succeeded in isolating bacteria closely related to Corynebacterium glutamicum, Chryseomicrobium aureum, and Enterococcus sp. for the first time. Anthraquinone was found to be an effective mediator that facilitated the indigo-reduction activity of the isolated strains. On analysis of the microbiota in indigo-fermentation suspensions, the ratio of indigo-reducing bacteria and others was found to be important for maintaining the indigo-reduction activity.