Rotatable Reagent Cartridge for High-Performance Microvalve System on a Centrifugal Microfluidic Device
Rotatable Reagent Cartridge for High-Performance Microvalve System on a Centrifugal Microfluidic Device
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DOI：
10.1021/ac400667e
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发表时间：
2013-07-16
影响因子：
7.4
通讯作者：
Yoshida, Yasukazu
中科院分区：
文献类型：
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作者：
Kawai, Takayuki;Naruishi, Nahoko;Yoshida, Yasukazu
近年来，微流控Lab-on-a-CD(LabCD)技术以其无泵、紧凑、芯片包含式的现场生物检测潜力引起了研究人员的广泛关注。为了控制LabCD中的流体，采用了微型阀，如毛细管阀、疏水性阀、虹吸阀和牺牲阀。然而，没有微型阀可以调节一个以上的通道。在具有许多顺序混合、洗涤和浪费步骤的复杂生物检测中，因此需要具有许多微通道、微阀和储液器的复杂的流体网络，这就增加了系统开发和芯片制备方面的检测成本。为了解决这个问题，我们开发了一种可旋转试剂盒(RRC)，它是一个柱状的槽，有几个房间来存储不同的试剂。通过简单的机械力将RRC嵌入并旋转LabCD中，只注入与下一个通道相连的房间中的试剂。通过调节RRC与LabCD的夹角，可以切换每种试剂的保存和喷射。我们开发的RRC没有通气孔，这是通过RRC的瓶子和盖子之间的透气间隙实现的。RRC可注入230nL-10亩L试剂，回收率达96%以上。最后，我们展示了一种L-乳酸的酶促分析方法，该方法可以很好地减少阀门和储液器的数量，显著简化了流体系统并提高了通道的集成性。用R-2>；0.999可以很好地对0-100万亩的L乳酸盐进行定量分析，这表明了RRC在微流控生物分析中的实用价值。
Recently, microfluidic lab-on-a-CD (LabCD) has attracted attentions of researchers for its potential for pumpless, compact, and chip-inclusive on-site bioassay. To control the fluids in the LabCD, microvalves such as capillary, hydrophobic, siphon, and sacrificial valves have been employed. However, no microvalve can regulate more than one channel. In a complicated bioassay with many sequential mixing, washing, and wasting steps, thus, an intricate fluidic network with many microchannels, microvalves, and reservoirs is required, which increases assay costs in terms of both system development and chip preparation. To address this issue, we developed a rotatable reagent cartridge (RRC), which was a column-shaped tank and has several rooms to store different reagents. By embedding and rotating the RRC in the LabCD with a simple mechanical force, only the reagent in the room connected to the following channel was injected. By regulating the angle of the RRC to the LabCD, conservation and ejection of each reagent could be switched. Our developed RRC had no air vent hole, which was achieved by the gas-permeable gap between the bottle and cap parts of the RRC. The RRC could inject 230 nL-10 mu L of reagents with good recoveries more than 96%. Finally, an enzymatic assay of L-lactate was demonstrated, where the number of valves and reservoirs were well minimized, significantly simplifying the fluidic system and increasing the channel integratability. Well quantitative analyses of 0-100 mu M L-lactate could easily be carried out with R-2 > 0.999, indicating the practical utility of the RRC for microfluidic bioanalysis.