Posttranscriptional down-regulation of small ribosomal subunit proteins correlates with reduction of 18S rRNA in RPS19 deficiency
Posttranscriptional down-regulation of small ribosomal subunit proteins correlates with reduction of 18S rRNA in RPS19 deficiency
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DOI：
10.1016/j.febslet.2009.05.023
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发表时间：
2009-06-18
期刊：
影响因子：
3.5
通讯作者：
Dahl, Niklas
中科院分区：
文献类型：
--
作者：
Badhai, Jitendra;Frojmark, Anne-Sophie;Dahl, Niklas
核糖体蛋白S19（RPS 19）在Diamond-Blackfan贫血（DBA）患者中发生突变。我们假设，RPS 19水平的降低导致其他核糖体（r-）蛋白在亚基水平的协调下调。我们发现，与大亚基（LSU）r-蛋白（L3，L9，L30和L38）相比，RPS 19的小干扰RNA（siRNA）敲低导致小亚基（SSU）r-蛋白（S20，S21和S24）相对减少。这与18 S rRNA相对于28 S rRNA的相对减少相关。R-蛋白mRNA水平保持相对不变，表明在亚基形成水平上对R-蛋白进行转录后调节。(C)2009年欧洲生物化学学会联合会。由Elsevier B出版。V.保留所有权利。
Ribosomal protein S19 (RPS19) is mutated in patients with Diamond-Blackfan anemia (DBA). We hypothesized that decreased levels of RPS19 lead to a coordinated down-regulation of other ribosomal (r-) proteins at the subunit level. We show that small interfering RNA (siRNA) knock-down of RPS19 results in a relative decrease of small subunit (SSU) r-proteins (S20, S21 and S24) when compared to large subunit (LSU) r-proteins (L3, L9, L30 and L38). This correlates with a relative decrease in 18S rRNA with respect to 28S rRNA. The r-protein mRNA levels remain relatively unchanged indicating a post transcriptional regulation of r-proteins at the level of subunit formation. (C) 2009 Federation of European Biochemical Societies. published by Elsevier B. V. All rights reserved.